Anti-SULT1A1 Antibody

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Anti-SULT1A1 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-SULT1A1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human SULT1A1
No cross-reactivity in ELISA with
Human SULT2A1
Human SULT2B1
E.coli cell lysate
Immunogen
Recombinant Human SULT1A1 protein (Catalog#11658-H07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SULT1A1 (rh SULT1A1; Catalog#11658-H07E; P50225-1; Glu 2-Leu 295). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #3C5H6
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-SULT1A1 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:1000-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-SULT1A1 Antibody (Mouse Monoclonal antibody) Images

Anti-SULT1A1 mouse monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:34 kDa

Observed band size:34 kDa

SULT1A1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-SULT1A1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SULT1A1 mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 34 kDa

Observed band size: 34 kDa

Anti-SULT1A1 Antibody: Alternative Names

Anti-HAST1/HAST2 Antibody; Anti-P-PST Antibody; Anti-PST Antibody; Anti-ST1A1 Antibody; Anti-ST1A3 Antibody; Anti-STP Antibody; Anti-STP1 Antibody; Anti-TSPST1 Antibody

SULT1A1 Background Information

Sulfate conjugation catalyzed by cytosolic sulfotransferase (SULT) enzymes. The SULTs are Phase II drug-metabolizing enzymes that catalyze the addition of a sulfuryl moiety to both endogenous compounds, including steroids and neurotransmitters, and certain xenobiotics, including N-hydroxy-2-acetylaminoflourine and phenolic compounds, like alpha-naphthol. SULTs may be involved in the individual genetic disposition, species differences, and organotropisms for toxicological effects of chemicals. Particularly SULT1A1 (Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1), a member of the sulfotransferase 1 subfamily, which is a major pathway for drug metabolism in humans. Humans have at least 1 functional SULT genes. There has been an explosion in information on sulfotransferase polymorphisms and their functional consequences. An Arg213His polymorphism in SULT1A1 has a strong influence on the level of enzyme protein and activity in platelets, which have been widely used for phenotyping. Statistically significant associations were observed between the SULT1A1 genotype (Arg213His) and age, obesity and certain neoplasias (mammary, pulmonary, esophageal and urothelial cancer). Furthermore, the polymorphism of the SULT1A1 may be closely associated with breast cancer.
Full Name
sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
References
  • Coughtrie MW. 2002. Sulfation through the looking glass--recent advances in sulfotransferase research for the curious. Pharmacogenomics J. 2(5): 297-308.
  • Klaassen CD, et al. (1998) Regulation of sulfotransferase mRNA expression in male and female rats of various ages. Chem Biol Interact. 109(1-3): 299-313.
  • Glatt H. (2000) Sulfotransferases in the bioactivation of xenobiotics. Chem Biol Interact. 129(1-2): 141-70.
  • Glatt H, et al. (2004) Pharmacogenetics of soluble sulfotransferases (SULTs). Naunyn Schmiedebergs Arch Pharmacol. 369(1): 55-68.
  • Hebbring SJ, et al. (2008) Sulfotransferase gene copy number variation: pharmacogenetics and function. Cytogenet Genome Res. 123(1-4): 205-10.

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