Mammalian Transient Protein Expression Service

Mammalian Transient Protein Expression Service Features

Mammalian cells are capable of protein folding and post-translational modification, which can express proteins with molecular structures, physical and chemical structures, and biological functions mostly approaching natural higher-orgainism proteins. It brings more possibility to obtain the same bioactivity as natural protein. Sino Biological's mammalian transient expression system, mainly using using CHO cells and HEK293 cells as expression hosts, can provide flexible services which could meet different custom needs, including tag removal, endotoxin control, SEC-HPLC assay, glycosylation analysis and so on.

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Mammalian Transient Protein Expression Service Details

Service procedures Specification Timeline Deliverables Pricing

Gene synthesis and codon optimization (optional)
  1-2 weeks  

Vector construction
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
1-2 weeks • Sequencing report

Expression and purification
• transient transfection of HEK293/CHO cells
protein purification
• SDS-PAGE and UV analysis
2-3 weeks • Feasibility report
• 0.1-0.5 mg sample
(If expression is feasible and sample remains from QC testing)

Large-scale expression and purification (optional)
  2-3 weeks • Purified protein
• SDS-PAGE report
• CoA

Case studies of Mammalian Transient Protein Expression Service

Case study of HEK293 Transient Protein A Expression

  • SDS-PAGE >98.7%
  • SEC-HPLC >98.2%
  • Bioactivity-ELISA: Protein A EC50=7.8 ng/mL
    Protein B EC50=6.8 ng/mL

Mammalian Transient Protein Expression Service Advantages

  • High-efficiency expression vectors
  • Years of experience of protein expression
  • Optimized mammalian expression systems
  • One-stop service from gene sequence to purified protein
  • High-throughput and large-scale production
  • High yield expression with post-translational modification and correct folding

Mammalian Transient Protein Expression Service Procedures

Communicate about protein expression requirements
Sign contract and make prepayment
Carry out pilot study for protein expression
Feedback pilot study result and ship sample (if feasible)
Bulk production quotation
Bulk production based on customer requirements

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