Custom Recombinant Protein Expression Services

Why choose Sino Biological for your protein production service?

• Sino has over 10 years' experience resulting in the successful development and production of 6000+ recombinant proteins.
• One-stop service from gene to protein
Multiple protein expression systems available including bacteria, yeast, baculovirus-insect and mammalian expression systems
• Multiple purification systems (30+) from which to choose
• Comprehensive research and development capabilities with over 1000 new proteins  generated per year
• Almost 100 bioreactors with various volume capacities ranging from 2 to 1000 L to meet high-throughput and large-scale production requirements

Overview of Recombinant Protein Expression: Key Influencing Factors, Case Studies and FAQs
Download Sino Biological's newest Brochure (2019):
Strategies to Obtain High-Quality Recombinant Proteins: Protocols and Case Studies

All protein production services

  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Expression evaluation
    • Protein expression and purification
    • Large-scale expression and purification (optional)
  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Expression and purification
    • Large-scale expression and purification (optional)
  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Virus packaging and amplification
    • Expression and purification
    • Large-scale expression and purification (optional)

Case Studies of Recombinant Protein Production Service Case Studies

Case study of E.coli protein A expression     More details>>>

  • SEC-HPLC >98.5%
  • Cell bioactivity: EC50=17pg/mL

Case study of Baculovirus-Insect protein A expression     More details>>>

  • SEC-HPLC >93%
  • Cell bioactivity: EC50=7ng/mL

Case study of HEK293 transient protein A expression     More details>>>

  • SEC-HPLC >98.2%
  • SDS-PAGE >98.7%

Custom Recombinant Protein Production Service Advantages

E.coli Protein Expression Service

• Over 10 years' experience of protein expression
• Multiple protein purification and refolding technologies
• High-throughput and large-scale protein expression and production
• One-stop service from gene to purified protein
• Multiple expression vectors and E.coli cells to meet various expression needs

Mammalian Transient Protein Expression Service

• High-efficiency expression vectors
• Optimized mammalian expression systems
• High-throughput and large-scale production
• High yield expression with post-translational modification and correct folding
• One-stop service from gene sequence to purified protein

Baculovirus-Insect Cell Protein Expression Service

• High-efficiency expression of intracellular and extracellular proteins
• Multiple insect cells: Sf9,Sf21,Hi-5
• High-throughput and large-scale protein production
• Optimized expression vectors and higher-titre virus packaging

Recombinant Protein Expression Systems

Bacterial, yeast, insect and mammalian protein expression systems are widely used. Each system has its advantages and challenges. For successful recombinant protein expression, it is important to choose the right system for a specific application. Protein type, function and desired protein yield will also influence the choice of the system.

Expression Systems E.coli Yeast Baculovirus-insect Mammalian
Prokaryotic Eukaryotic Eukaryotic Eukaryotic
Advantages • Low cost
• Rapid expression
• Easy scale-up
• Most widely used system for recombinant protein production
• Low cost 
• Rapid expression 
• High yield
• Diverse post-translational modifications
• High capacity genes 
• Soluble proteins
• Suitable for toxic proteins
• Post-translational modifications similar to mammalian systems
• Soluble protein
• Lower endotoxin 
• Better bioactivity
• Comprehensive post-translational modifications 
• Transient & stable expression
Challenges • Inclusion bodies
• Lack of eukaryotic post-translational modifications
• Difficult to express higher MW proteins
• Improper glycosylation 
• High mannose modification
• More demanding culture conditions
• Lack of partial glycosylation
• More demanding culture conditions
Most Common Applications • Bacterial proteins
• Antigen proteins
Cytokines
• Enzymes
Cytokines
• Lower MW proteins
• Enzymes
• Cytoplasmic proteins 
• Toxic proteins
• Transmembrane proteins
• Secretory proteins
Kinases
• Secretory proteins
• Extracellular domain of transmembrane proteins
Recombinant antibodies
• antibody fragments

Contact Us

Tel
+86-010-50911666
Fax
+86-010-50953280
Email
cro-service@sinobiological.com