Recombinant antibody production is a key technology for the industrialization of monoclonal antibodies. Without immunization and screening process, antibody gene sequence can be directly & stably used to manufacture large quantities of antibodies. Sino Biological has a complete recombinant antibody expression & production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.
Service procedures | Specification | Timeline | Deliverables | Pricing |
---|---|---|---|---|
① Gene synthesis and codon optimization (optional) |
1-2 weeks | • Purified antibody • SDS-PAGE data • CoA |
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② Vector construction |
• Cloning the HC and LC gene into optimized expression vectors, respectively • Plasmid sequencing • Large scale plasmid production |
1-2 weeks | ||
③ Expression & purification |
• Transient transfection of HEK293/CHO cells • Antibody purification • Polishing if needed |
1-2 weeks | ||
④ QC analysis |
• Analysis by SDS-PAGE and UV • Purity analysis by HPLC-SEC and endotoxin detection (optional) |
3 days |
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bd@sinobiological.comFor CRO Services
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