Large-scale Antibody Production Service

Large-scale Antibody Production Service Introduction

Recombinant antibody production is a key technology for the industrialization of monoclonal antibodies. Without immunization and screening process, antibody gene sequence can be directly & stably used to manufacture large quantities of antibodies. Sino Biological has a complete recombinant antibody expression & production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.

More Antibody Production & Development ServicesQuote!

Large-scale Antibody Production Service Process

Service procedures Specification Timeline Deliverables Pricing

Gene synthesis and codon optimization (optional)
  1-2 weeks • Purified antibody
• SDS-PAGE data
• CoA

Vector construction
• Cloning the HC and LC gene into optimized expression vectors, respectively
• Plasmid sequencing
• Large scale plasmid production
1-2 weeks

Expression & purification
• Transient transfection of HEK293/CHO cells
• Antibody purification
• Polishing if needed
1-2 weeks

QC analysis
• Analysis by SDS-PAGE and UV
• Purity analysis by HPLC-SEC and endotoxin detection (optional)
3 days
In this large-scale antibody production service, customers need to provide:Antibody gene sequence or vectors containing antibody gene

Large-scale Antibody Production Service Advantages

  • Self-developed high-efficiency culture medium and transfection reagents, high-expression vectors and engineering cell lines with indigenous intellectual property, ensuring high success rates at low cost for antibody production.
  • Different culture scale from shake flasks to bioreactors, 24 hours a day, 7 days a week continuous culturing to ensure rapid production of antibodies.
  • Provide transient transfection for antibody production, and develop stable cell lines for antibody expression to meet different customers needs.
  • Express full-length antibody of each subtype (IgG1, IgG2a, IgG2b, IgG3, IgG4, IgM, etc.), chimeric antibody (human/mouse chimeric antibody, mouse/rabbit chimeric antibody, etc.), and also antibody fragments (Fab, scFv, nanobody, etc.).
  • Manufacture antibodies with high-purity, high-concentration and low-endotoxin according to customers demands.
  • One-stop Service: mAb development- hybridoma antibody sequencing - mouse antibody production - chimeric antibody production - antibody humanization - antibody affinity maturation - antibody characterization and analysis- process development - GMP production.

Contact Us