Large-scale Antibody Production Service
Large-scale Antibody Production Service Introduction
Recombinant antibody production is a key technology for the industrialization of monoclonal antibodies. Without immunization and screening process, antibody gene sequence can be directly & stably used to manufacture large quantities of antibodies. Sino Biological has a complete recombinant antibody expression & production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.
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Large-scale Antibody Production Service Process
| Service procedures | Specification | Timeline | Deliverables | Pricing |
|---|---|---|---|---|
| ① Gene synthesis and codon optimization (optional) |
1-2 weeks | • Purified antibody • SDS-PAGE data • CoA |
||
| ② Vector construction |
• Cloning the HC and LC gene into optimized expression vectors, respectively • Plasmid sequencing • Large scale plasmid production |
1-2 weeks | ||
| ③ Expression & purification |
• Transient transfection of HEK293/CHO cells • Antibody purification • Polishing if needed |
1-2 weeks | ||
| ④ QC analysis |
• Analysis by SDS-PAGE and UV • Purity analysis by HPLC-SEC and endotoxin detection (optional) |
3 days |
In this large-scale antibody production service, customers need to provide:Antibody gene sequence or vectors containing antibody gene
Large-scale Antibody Production Service Advantages
- Self-developed high-efficiency culture medium and transfection reagents, high-expression vectors and engineering cell lines with indigenous intellectual property, ensuring high success rates at low cost for antibody production.
- Different culture scale from shake flasks to bioreactors, 24 hours a day, 7 days a week continuous culturing to ensure rapid production of antibodies.
- Provide transient transfection for antibody production, and develop stable cell lines for antibody expression to meet different customers needs.
- Express full-length antibody of each subtype (IgG1, IgG2a, IgG2b, IgG3, IgG4, IgM, etc.), chimeric antibody (human/mouse chimeric antibody, mouse/rabbit chimeric antibody, etc.), and also antibody fragments (Fab, scFv, nanobody, etc.).
- Manufacture antibodies with high-purity, high-concentration and low-endotoxin according to customers demands.
- One-stop Service: mAb development- hybridoma antibody sequencing - mouse antibody production - chimeric antibody production - antibody humanization - antibody affinity maturation - antibody characterization and analysis- process development - GMP production.
