Compared with the ascites method for production of mAb, hybridoma cells can be cultured and purified in vitro, which has the advantages of high purity, high batch-to-batch reproducibility, less impurity and no animal virus contamination. Sino Biological provides hybridoma cell culture and antibody production services which can use low-serum or serum-free media for high-density culture in vitro and reduce bovine IgG contamination. And it has been a preferred option for domestic and foreign customers to produce antibodies using hybridoma cells.
|• Cell recovery and cell counting||1-2 days||• Purified antibody
• SDS-PAGE data
|• Cells adaption into serum-free or low-serum growth
• Fed-batch process development
Production & purification
|• Appropriate scale hybridoma cell culture
• Purification of the antibody by protein A / G affinity column
|• Analysis by SDS-PAGE and UV||3 days|
1.Provide at least 2 tubes of frozen hybridoma cells or two T25 of living cells, number of cells >1 x 10^6.
2.Provide the information of antibody subtypes.