Compared with the ascites method for production of mAb, hybridoma cells can be cultured and purified in vitro, which has the advantages of high purity, high batch-to-batch reproducibility, less impurity and no animal virus contamination. Sino Biological provides hybridoma cell culture and antibody production services which can use low-serum or serum-free media for high-density culture in vitro and reduce bovine IgG contamination. And it has been a preferred option for domestic and foreign customers to produce antibodies using hybridoma cells.
| Service procedures | Specification | Timeline | Deliverables |
|---|---|---|---|
| ① Cell recovery |
• Cell recovery and cell counting | 1-2 days | • Purified antibody • SDS-PAGE data • CoA |
| ② Adaptation |
• Cells adaption into serum-free or low-serum growth • Fed-batch process development |
4-6 weeks | |
| ③ Production & purification |
• Appropriate scale hybridoma cell culture • Purification of the antibody by protein A / G affinity column |
6-10 weeks | |
| ④ QC analysis |
• Analysis by SDS-PAGE and UV | 3 days |
1.Provide at least 2 tubes of frozen hybridoma cells or two T25 of living cells, number of cells >1 x 10^6.
2.Provide the information of antibody subtypes.
