Owing to their advantages of high specificity and stability, mouse monoclonal antibodies (mouse mAbs) have been widely used in basic research as well as in medical diagnosis. However, mouse monoclonal antibodies produced by hybridoma technique requires a long time (about 15-20 weeks) to obtain a qualified antibody and animal immunization is the most time-consuming, usually 8-12 weeks. In order to satisfy customers' urgent needs for mouse monoclonal antibody, Sino Biological has shortened the immunization time to 4-5 weeks and launched Fast Mouse mAb Production Service, which can offer high quality antibodies more quickly and efficiently.
| Service procedures | Specification | Timeline | Deliverables | Guarantee | Pricing |
|---|---|---|---|---|---|
| ① Antigen preparationQuote！ |
Please refer to antigen production service | • Positive hybridomas • Purified antibody • 2 tubes of frozen cells per clone • CoA |
An ELISA positive result against the immunogen | ||
| ② Immunization and serum titer test |
• Pre-immune bleed • Immunization of five Balb/c mice • Serum titer test • Final bleed |
5 weeks | |||
| ③ Fusion and screening |
• Fusion and screening • Subcloning and cell expansion • Cell Banking |
5 weeks | |||
| ④ Antibody production and analysis |
• Hybridoma cell culture at a proper scale • Purified by protein A affinity chromatography • Analysis by SDS-PAGE and UV • ELISA validation |
3 weeks |
