Baculovirus-Insect Cell Protein Expression Service

Baculovirus-Insect Cell Protein Expression Service Introduction

Baculovirus-insect cell system belongs to eukaryotic expression system, with capability of protein folding and modification after protein translation. It is suitable for expressing proteins that are probably harmful to mammalian host cells, such as kinases and toxic proteins. Sino Biological possesses abundant experience of baculovirus-Insect cell protein expression, especially the expression of large proteins, toxic proteins, kinases, intracellular proteins and membrane proteins.

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Baculovirus-Insect Cell Protein Expression Service Items

Service procedures Specification Timeline Deliverables Pricing

Gene synthesis and codon optimization (optional)
  1-2 weeks • Sequencing report

Vector construction
• Clone cDNA into expression vector
• Plasmid sequencing
• Large-scale plasmid preparation
1-2 weeks

Virus packaging and amplification
• Virus packaging
• Virus amplification
2-3 weeks

Expression and purification
• Virus infecting cells
• Small expression, purification and condition optimization
• SDS-PAGE and UV analysis
2-3 weeks • Feasibility report
• 0.1-0.5 mg sample
(If expression is feasible and sample remains from QC testing)

Large-scale expression and purification (optional)
  3-5 weeks • Purified protein
• SDS-PAGE report
• CoA

Baculovirus-Insect Cell Protein Expression Service Procedures

Communicate about protein expression requirements
Sign contract and make prepayment
Carry out pilot study for protein expression
Feedback pilot study result and ship sample (if feasible)
Bulk production quotation
Bulk production based on customer requirements

Case Presentation of Baculovirus-Insect Cell Protein Expression Service

Protein A:

  • 1. SDS-PAGE >97%
  • 2. SEC-HPLC >93%
  • 3. Cell-based activity assay:EC50=7ng/mL

Baculovirus-Insect Cell Protein Expression Service Advantages

  • Years of experience of protein expression
  • One-stop service from gene sequence to purified protein
  • High-efficiency expression of intracellular and extracellular proteins
  • Multiple insect cells: Sf9, Sf21, Hi-5
  • High-throughput and large-scale protein production
  • Optimized expression vectors and higher-titer virus packaging

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