TIE2 Protein Overview: Sequence, Structure, Function and Protein Interaction

TIE2 Protein Overview

TIE2 protein family

Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

TIE2 protein name

Recommended name
Angiopoietin-1 receptor
Aliases
CD202b, TIE2, TIE-2, VMCM1
Alternative name
Endothelial tyrosine kinase Tunica interna endothelial cell kinase Tyrosine kinase with Ig and EGF homology domains-2 Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 p140 TEK CD_antigen: CD202b

TIE2 Gene family protein

TIE2 Protein Sequence

Species Human TIE2 protein
Length 1124
Mass (Da) 125830
Sequence Human TIE2 protein sequence
Species Mouse TIE2 protein
Length 1122
Mass (Da) 125701
Sequence Mouse TIE2 protein sequence
Species
Length
Mass (Da)
Sequence

TIE2 Protein Molecular Weight & PI

Angiopoietin-1 receptor precursor (EC 2.7.10.1) (Endothelial tyrosine kinase) (Tunica interna endothelial cell kinase) (Tyrosine kinase with Ig and EGF homology domains-2) (Tyrosine-protein kinase receptor TEK) (Tyrosine-protein kinase receptor TIE-2) (hTIE2) (p140 TEK) (CD202b antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 23-1124 Angiopoietin-1 receptor.

Molecular weight (Da)

123683.48

Theoretical pI

6.61

TIE2 Protein Structure

TIE2 KINASE DOMAIN
Deposited
2000-09-20   Released:  2001-09-20
Deposition Author(s)
Shewchuk, L.M., Hassell, A.M., Ellis, B., Holmes, W.D., Davis, R., Horne, E.L., Kadwell, S.H., McKee, D.D., Moore, J.T.
Organism(s)
Homo sapiens
Expression System
unidentified baculovirus
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.2000 Å
R-Value Free
0.225
R-Value Work
0.225
1FVR From PDB

Human TIE2 protein Secondary structure

TIE2 Protein Interaction

Recombinant TIE2 Protein Feature

TIE2 Protein, Human, Recombinant (ECD, His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its binding ability in a functional ELISA . Immobilized recombinant human Tie2 at 2 μg/ml (100 μl/well) can bind human Angiopoietin-2 at a linear range of 1.28-160 ng/ml.

Recombinant TIE2 protein citations

Title
Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy
Year
2017
Author
Shlamkovich, T;Aharon, L;Barton, WA;Papo, N;
Journal
Oncotarget
Title
Identification of specific Tie2 cleavage sites and therapeutic modulation in experimental sepsis
Year
2020
Author
Idowu, TO;Etzrodt, V;Seeliger, B;Bolanos-Palmieri, P;Thamm, K;Haller, H;David, S;
Journal
Elife

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