Neuropilin-1 Protein

Neuropilin-1 Protein Overview

Neuropilin-1 reagents

NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; 192240) and semaphorin (see SEMA3A; 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

Neuropilin-1 protein family

Belongs to the neuropilin family.

Neuropilin-1 protein name

Recommended name
Neuropilin-1
Aliases
CD304, NRP, VEGF165R
Alternative name
Vascular endothelial cell growth factor 165 receptor CD_antigen: CD304

Neuropilin-1 Protein Sequence

Species Human Neuropilin-1 protein
Length 923
Mass (Da) 103134
Sequence Human Neuropilin-1 protein sequence
Species Mouse Neuropilin-1 protein
Length 923
Mass (Da) 103000
Sequence Mouse Neuropilin-1 protein sequence
Species Rat Neuropilin-1 protein
Length 922
Mass (Da) 103082
Sequence Rat Neuropilin-1 protein sequence

Neuropilin-1 Protein Molecular Weight & PI

Neuropilin-1 precursor (Vascular endothelial cell growth factor 165 receptor) (CD304 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 22-923 Neuropilin-1.

Molecular weight (Da)

101073.76

Theoretical pI

5.58

Neuropilin-1 Protein Structure

X-ray structure of neuropilin-1 b1 domain complexed with Arg-5 ligand.
Deposited
2016-03-29   Released:  2017-04-05
Deposition Author(s)
Fotinou, C., Rana, R., Djordjevic, S., Yelland, T.
Organism(s)
Homo sapiens
Expression System
Escherichia coli BL21(DE3)
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.1000 Å
R-Value Free
0.223
R-Value Work
0.182
5J1X From PDB

Human Neuropilin-1 protein Secondary structure

Neuropilin-1 Protein Interaction

Recombinant Neuropilin-1 Protein Feature

Neuropilin-1 Protein, Human, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
1. Using the Octet RED System, the affinity constant (Kd) of NRP1-his bound to biotinylated human VEGF165 was 25nM. 2. Measured by its binding ability in a functional ELISA. Immobilized NRP1-His (Cat:10011-H08H) at 10 μg/mL (100 μL/well) can bind VEGF165/Biotin (Cat:11066-HNAH), the EC50 of VEGF165/Biotin (Cat:11066-HNAH) is 18-40 ng/mL.

Recombinant Neuropilin-1 protein citations

Title
Evidence for Pro-angiogenic Functions of VEGF-Ax
Year
2016
Author
Xin, H;Zhong, C;Nudleman, E;Ferrara, N;
Journal
Cell
Title
Neuropilin-1 drives tumor-specific uptake of chlorotoxin
Year
2019
Author
McGonigle, S;Majumder, U;Kolber-Simonds, D;Wu, J;Hart, A;Noland, T;TenDyke, K;Custar, D;Li, D;Du, H;Postema, MHD;Lai, WG;Twine, NC;Woodall-Jappe, M;Nomoto, K;
Journal
Cell Commun. Signal

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