LIF Antibody Guideline: Preparation, Dilution and Applications

LIF Antibody Overview

There are 5 LIF antibodies which are validated in multiple tissues with various applications, including ELISA. There are 5 LIF antibody for ELISA. Among all these LIF antibodies, there are 1 anti-LIF mouse monoclonal antibodies , 4 anti-LIF rabbit polyclonal antibodies . All the LIF anbodies are produced in house and all are in stock. LIF antibody customerized service is available.

LIF Antibody ELISA

LIF antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Leukemia Inhibitory Factor/LIF (rh Leukemia Inhibitory Factor/LIF; Catalog#14890-H08H; P15018; Met1-Phe202). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

LIF antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

LIF Antibody western blot

LIF Antibody western blot

LIF Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse LIF (Catalog#50755-M08H; P09056; Met1-Phe203). LIF specific IgG was purified by Mouse LIF affinity chromatography.

LIF Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

LIF Antibody immunoprecipitation

LIF Antibody immunoprecipitation

LIF Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Mouse LIF (Catalog#50755-M08H; P09056; Met1-Phe203). LIF specific IgG was purified by Mouse LIF affinity chromatography.

LIF Antibody immunoprecipitation Validated

Application IP
Dilution 1-2 μL/mg of lysate
Notes