IL-6R Protein Overview: Sequence, Structure, Function and Protein Interaction
IL-6R Protein Overview
Interleukin-6 (IL6; 147620) is a multifunctional cytokine that is essential to the regulation of the immune response, hematopoiesis, and acute-phase reactions. It exerts its many actions through a heterodimeric receptor consisting of 2 membrane-bound glycoproteins: an 80-kD IL6-binding subunit, IL6R-alpha, and gp130 (IL6ST; 600694), which is responsible for signal transduction and stabilization of the alpha-chain ligand complex. Yamasaki et al. (1988) isolated a cDNA encoding the receptor for interleukin-6. They showed that it codes for a protein consisting of 468 amino acids, including a signal peptide of about 19 amino acids and a domain of about 90 amino acids that is similar to a domain in the immunoglobulin superfamily. The cytoplasmic domain of about 82 amino acids lacks a tyrosine/kinase domain, unlike other growth factor receptors.
IL-6R protein family
Belongs to the type I cytokine receptor family. Type 3 subfamily.
IL-6R protein name
Recommended name
Interleukin-6 receptor subunit alpha
Short name
IL-6 receptor subunit alpha||IL-6R subunit al
Aliases
CD126
Alternative name
IL-6R 1
Membrane glycoprotein 80
CD_antigen: CD126
IL-6R Gene family protein
CD molecules
| Approved symbol | Common name |
|---|---|
| IFNGR1 | IFNGR1 protein, recombinant |
| TEK | TIE2 protein, recombinant |
| CD274 | PD-L1 protein, recombinant |
| ICOS | ICOS protein, recombinant |
| TNFRSF21 | DR6 protein, recombinant |
| SDC2 | Syndecan-2 protein, recombinant |
| PTPRC | CD45 protein, recombinant |
| ANPEP | CD13 protein, recombinant |
| FZD4 | Frizzled 4 protein, recombinant |
| CD63 | CD63 protein, recombinant |
| TNFSF8 | CD30L protein, recombinant |
| TFRC | Transferrin Receptor/TFRC protein, recombinant |
| CD82 | CD82 protein, recombinant |
| CD36 | CD36 protein, recombinant |
| CD34 | CD34 protein, recombinant |
| CEACAM8 | CD66b protein, recombinant |
| SLAMF7 | SLAMF7/CD319 protein, recombinant |
| TNFRSF1B | TNFR2/TNFRSF1B protein, recombinant |
| FASLG | Fas Ligand protein, recombinant |
| F11R | JAM-A/Junctional Adhesion Molecule A protein, recombinant |
Immunoglobulin like domain containing
| Approved symbol | Common name |
|---|---|
| CXADR | CAR/CXADR protein, recombinant |
| AXL | AXL protein, recombinant |
| IL18RAP | IL18RAP protein, recombinant |
| KIRREL1 | KIRREL protein, recombinant |
| FCMR | FCMR/FAIM3 protein, recombinant |
| FCGR3B | CD16b protein, recombinant |
| NPTN | NPTN protein, recombinant |
| LAIR1 | LAIR1 protein, recombinant |
| CD84 | CD84 protein, recombinant |
| MERTK | Mer protein, recombinant |
| FCGR2B | CD32B/Fcgr2b protein, recombinant |
| CD96 | CD96 protein, recombinant |
| IGSF3 | IGSF3 protein, recombinant |
| CADM4 | CADM4 protein, recombinant |
| THY1 | Thy1/CD90 protein, recombinant |
| TEK | TIE2 protein, recombinant |
| CEACAM6 | CEACAM6 protein, recombinant |
| JAM3 | JAM-C protein, recombinant |
| IL18BP | IL18BP protein, recombinant |
| CNTN3 | Contactin 3 protein, recombinant |
Interleukin receptors
| Approved symbol | Common name |
|---|---|
| IL5RA | IL5RA protein, recombinant |
| IL12RB2 | IL12RB2 protein, recombinant |
| IL1RAPL1 | IL1RAPL1 protein, recombinant |
| IL1RL1 | ST2/IL-1 RL1 protein, recombinant |
| IL17RC | IL17RC protein, recombinant |
| IL20RB | IL20RB protein, recombinant |
| IL2RG | IL2RG protein, recombinant |
| IL13Ra1 | IL13RA1 protein, recombinant |
| IL1R1 | IL1R1 protein, recombinant |
| IL4R | IL4R protein, recombinant |
| IL21R | IL21 Receptor protein, recombinant |
| IL2RA | CD25/IL2RA protein, recombinant |
| IL12RB1 | IL12RB1 protein, recombinant |
| IL31RA | IL31RA protein, recombinant |
| IL2RB | CD122/IL2RB protein, recombinant |
| IL1RN | IL1RA protein, recombinant |
| IL17RA | IL17RA protein, recombinant |
| IL1RL2 | IL1RL2 protein, recombinant |
| IL18RAP | IL18RAP protein, recombinant |
| IL27Ra | TCCR protein, recombinant |
IL-6R Protein Sequence
| Species | Human IL-6R protein |
|---|---|
| Length | 468 |
| Mass (Da) | 51548 |
| Sequence | Human IL-6R protein sequence |
| Species |
|---|
| Length |
| Mass (Da) |
| Sequence |
| Species | Rat IL-6R protein |
|---|---|
| Length | 462 |
| Mass (Da) | 50401 |
| Sequence | Rat IL-6R protein sequence |
IL-6R Protein Molecular Weight & PI
Interleukin-6 receptor subunit alpha precursor (IL-6 receptor subunit alpha) (IL-6R subunit alpha) (IL-6R-alpha) (IL-6RA) (IL-6R 1) (Membrane glycoprotein 80) (gp80) (CD126 antigen) Homo sapiens (Human).
The parameters have been computed for the following feature
FT CHAIN 20-468 Interleukin-6 receptor subunit alpha.
Molecular weight (Da)
49868.41
Theoretical pI
8.61
IL-6R Protein Structure
Biophysical and cellular characterisation of a junctional epitope antibody that locks IL-6 and gp80 together in a stable complex: implications for new therapeutic strategies
Deposited
2016-01-25 Released: 2017-01-25
Deposition Author(s)
Adams, R., Griffin, R., Doyle, C., Ettorre, A.
Organism(s)
Homo sapiens
Expression System
Escherichia coli, Homo sapiens
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.7000 Å
R-Value Free
0.287
R-Value Work
0.231
5FUC From PDB
Human IL-6R protein Secondary structure
IL-6R Protein Interaction
Recombinant IL-6R Protein Feature
IL-6R Protein, Human, Recombinant (ECD, His Tag)
High Purity
> 90 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/mL (100μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/ml.
2. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 20-80 ng/ml.
3. Using the Octet RED System, the affinity constant (Kd) of IL-6R Protein, Human, Recombinant (His Tag) (Cat. 10398-H08H) bound Anti-IL6R Antibody (Cat. 10398-MM08) was 0.1 nM.
Recombinant IL-6R protein citations
Title
Chikusetsusaponin IVa Butyl Ester (CS-IVa-Be), a Novel IL6R Antagonist, Inhibits IL6/STAT3 Signaling Pathway and Induces Cancer Cell Apoptosis
Year
2016
Author
Yang, J;Qian, S;Cai, X;Lu, W;Hu, C;Sun, X;Yang, Y;Yu, Q;Gao, SP;Cao, P;
Journal
Mol. Cancer Ther.
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