HGF Protein Overview: Sequence, Structure, Function and Protein Interaction

HGF Protein Overview

Plasma from patients with fulminant hepatic failure contains a factor that stimulates the growth of adult rat hepatocytes in primary culture. Gohda et al. (1988) purified HGF from a patient's plasma and showed that it has multiple forms with molecular mass between 76 and 92 kD. HGF consists of 2 chains, heavy and light, with molecular mass of 54 to 65 kD and 31.5 and 34.5 kD, respectively. These chains are linked together by disulfide bonds. Miyazawa et al. (1989) and Nakamura et al. (1989) sequenced cDNAs encoding HGF. The deduced sequence of the pre-pro-protein consisted of 728 amino acid residues. The sequence showed that the heavy and light chains are encoded by the same mRNA and are produced from a common translation product by proteolytic processing. The difference in deduced number of amino acid residues probably indicates that HGF is distinct from hepatocyte stimulatory factor (HSF), which is the same as interferon beta-2 (147620). Rubin et al. (1991) studied a mitogen derived from lung fibroblasts which, if not identical to HGF, is closely related to it. They showed that their human lung fibroblast-derived mitogen had a spectrum of targets including endothelial cells and melanocytes in addition to epithelial cells. Gherardi and Stoker (1990) found that HGF is structurally similar, if not identical, to scatter factor, a molecule shown to stimulate the dissociation and scattering of epithelial cells. The sequence of HGF has about 35% identity to that of plasminogen (173350) and the putative cleavage site of HGF is identical to that of plasminogen. A potent mitogen for hepatocytes, HGF is also known as hepatopoietin A (HBGA) (Szpirer et al., 1992). Weidner et al. (1991) presented structural and functional evidence that human scatter factor (SF) and human HGF are identical proteins encoded by a single gene. Lung fibroblast-derived mitogen is also coded by the same gene. The HGF cellular receptor gene, the MET oncogene (164860), is located on 7q with the HGF gene. Thus, cells with polysomy of chromosome 7 may simultaneously overproduce both the factor and its receptor and acquire invasive properties through an autocrine mechanism. An increase in the copy number of chromosome 7 is one of the most common chromosome abnormalities observed in human malignant gliomas (137800). Schultz et al. (2009) determined that HGF shares 88% identity with its mouse homolog. The authors stated that there are multiple isoforms of HGF. HGF isoform-1 encodes a preprotein that is cleaved into alpha and beta chains. The alpha chain is composed of a hairpin loop follow by 4 N-terminal kringle domains, and the beta chain has homology to trypsin-like serine proteases but has no catalytic function. HGF isoform-2 encodes only 2 kringle domains, and HGF isoform-3 and isoform-4 are similar to 1 and 2, respectively, differing only in the use of alternate exon 5b splice acceptor site. Isoform-5 encodes only 1 kringle domain and utilizes the exon 5a acceptor site. Schultz et al. (2009) identified 2 additional short isoforms of HGF including exons 1 to 4 and a 3-prime untranslated region transcribed from intron 4.

HGF protein family

Belongs to the peptidase S1 family. Plasminogen subfamily.

HGF protein name

Recommended name
Hepatocyte growth factor
Short name
SF
Aliases
fibroblast-derived tumor cytotoxic factor, F-TCF, hepatopoietin A, HGFB, HPTA, lung fibroblast-derived mitogen, scatter factor, SF
Alternative name
Hepatopoietin-A Scatter factor Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain

HGF Protein Sequence

Species Human HGF protein
Length 728
Mass (Da) 83134
Sequence Human HGF protein sequence
Species Mouse HGF protein
Length 728
Mass (Da) 82945
Sequence Mouse HGF protein sequence
Species Rat HGF protein
Length 728
Mass (Da) 82906
Sequence Rat HGF protein sequence

HGF Protein Molecular Weight & PI

Hepatocyte growth factor precursor (Hepatopoietin-A) (Scatter factor) (SF) [Contains: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 32-494 Hepatocyte growth factor alpha chain.

Molecular weight (Da)

53684.21

Theoretical pI

7.69

HGF Protein Structure

CRYSTAL STRUCTURES OF NK1-HEPARIN COMPLEXES REVEAL THE BASIS FOR NK1 ACTIVITY AND ENABLE ENGINEERING OF POTENT AGONISTS OF THE MET RECEPTOR
Deposited
2001-09-20   Released:  2001-10-02
Deposition Author(s)
Lietha, D., Chirgadze, D.Y., Mulloy, B., Blundell, T.L., Gherardi, E.
Organism(s)
Homo sapiens
Expression System
Komagataella phaffii GS115
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.0000 Å
R-Value Free
0.295
R-Value Work
0.254
1GMO From PDB

Human HGF protein Secondary structure

HGF Protein Interaction

Recombinant HGF Protein Feature

HGF Protein, Human, Recombinant

High Purity
> 93 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 0.5-5 ng/mL.

Recombinant HGF protein citations

Title
Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response
Year
2016
Author
TAN, X;JUAN, F;SHAH, AQ;
Journal
Journal of Zhejiang University Science B
Title
Rapid generation of functional hepatocyte-like cells from human adipose-derived stem cells
Year
2016
Author
Fu, Y;Deng, J;Jiang, Q;Wang, Y;Zhang, Y;Yao, Y;Cheng, F;Chen, X;Xu, F;Huang, M;Yang, Y;Zhang, S;Yu, D;Zhao, RC;Wei, Y;Deng, H;
Journal
Stem Cell Res Ther
Title
Chitosan-assisted differentiation of porcine adipose tissue-derived stem cells into glucose-responsive insulin-secreting clusters
Year
2017
Author
Liu, HY;Chen, CC;Lin, YY;Chen, YJ;Liu, BH;Wong, SC;Wu, CY;Chang, YT;Chou, HE;Ding, ST;
Journal
PLoS ONE
Title
Synergistically Controlled Stemness and Multilineage Differentiation Capacity of Stem Cells on Multifunctional Biointerfaces
Year
2017
Author
Wu, C;Liu, H;Huang, C;Yeh, S;Cheng, N;Ding, S;Chen, H;
Journal
Advanced Materials Interfaces
Title
Motor Neuron-Skeletal Muscle Co Culture Model: A Potential Novel in Vitro and Computaional Platform to Investigate Cancer Cachexia
Year
2018
Author
Al Samid, M;Al-Shanti, N;Odeh, M;
Journal
2018 1st International Conference on Cancer Care Informatics (CCI)
Title
A Short Isoform of Coagulation Factor XII mRNA Is Expressed by Neurons in the Human Brain
Year
2019
Author
Zamolodchikov, D;Bai, Y;Tang, Y;McWhirter, JR;Macdonald, LE;Alessandri-Haber, N;
Journal
Neuroscience
Title
Simplified in vitro engineering of functional mammalian neuromuscular junctions between embryonic rat motor neurons and immortalised human skeletal muscle cells
Year
2019
Author
Saini, J;
Journal
Thesis

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