Her2/ERBB2 Antibody Guideline: Preparation, Dilution and Applications

Her2/ERBB2 Antibody Overview

There are 39 Her2/ERBB2 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, ICC/IF, IHC-P, ELISA(Det), ELISA(Cap), WB, IP. There are 23 Her2/ERBB2 antibody for ELISA, 12 Her2/ERBB2 antibody for FCM, 6 Her2/ERBB2 antibody for ICC/IF, 8 Her2/ERBB2 antibody for IHC-P, 1 Her2/ERBB2 antibody for ELISA(Det), 1 Her2/ERBB2 antibody for ELISA(Cap), 9 Her2/ERBB2 antibody for WB, 2 Her2/ERBB2 antibody for IP. Among all these Her2/ERBB2 antibodies, there are 11 anti-Her2/ERBB2 mouse monoclonal antibodies , 10 anti-Her2/ERBB2 rabbit monoclonal antibodies , 18 anti-Her2/ERBB2 rabbit polyclonal antibodies . All the Her2/ERBB2 anbodies are produced in house and all are in stock. Her2/ERBB2 antibody customerized service is available.

Her2/ERBB2 Antibody ELISA

Her2/ERBB2 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ErbB2 / HER2 extracellular domain (rhErbB2; Catalog#10004-H08H; NP_004439.2; Met 1-Thr 652). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Her2/ERBB2 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Her2/ERBB2 Antibody western blot

Her2/ERBB2 Antibody western blot

Her2/ERBB2 Antibody western blot Preparation

Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human ERBB2 , and purified by antigen affinity chromatography.

Her2/ERBB2 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Her2/ERBB2 Antibody flow cytometry

Her2/ERBB2 Antibody flow cytometry

Her2/ERBB2 Antibody immunohistochemistry

Her2/ERBB2 Antibody immunohistochemistry

Her2/ERBB2 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HER2/ERBB2 (rh HER2/ERBB2; Catalog#10004-H20B1; NP_004439.2; Lys676-Val1255). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Her2/ERBB2 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:400-1:2000
Notes  

Her2/ERBB2 Antibody immunoprecipitation

Her2/ERBB2 Antibody immunoprecipitation

Her2/ERBB2 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human HER2 / ErbB2 (rh HER2 / ErbB2; Catalog#10004-H20B1; NP_004439.2; Lys676-Val1255). HER2 / ErbB2 specific IgG was purified by Human HER2 / ErbB2 affinity chromatography.

Her2/ERBB2 Antibody immunoprecipitation Validated

Application IP
Dilution 2-4 μL/mg of lysate
Notes  

Her2/ERBB2 Antibody ICC / IF

Her2/ERBB2 Antibody ICC / IF

Her2/ERBB2 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human ErbB2 / HER2 extracellular domain (rh ErbB2; Catalog#10004-H08H; NP_004439.2; Met 1-Thr 652).

Her2/ERBB2 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:50-1:1000
Notes  

Her2/ERBB2 antibody citations

Title
Development of multiplexed silicon dual microring sensor for the detection of bladder cancer markers
Year
2012
Author
Kim, KW;Song, J;Liu, Q;Kee, JS;Shin, Y;
Journal
Photonics Global Conference (PGC), 2012
Title
Isolation of scFv antibody fragments against HER2 and CEA tumor antigens from combinatorial antibody libraries derived from cancer patients
Year
2013
Author
Ayat, H;Burrone, OR;Sadghizadeh, M;Jahanzad, E;Rastgou, N;Moghadasi, S;Arbabi, M;
Journal
Biologicals
Title
Label-free biosensor based on an electrical tracing-assisted silicon microring resonator with a low-cost broadband source
Year
2013
Author
Kim, KW;Song, J;Kee, JS;Liu, Q;Lo, GQ;Park, MK;
Journal
Biosens Bioelectron
Title
Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD
Year
2014
Author
Marques, RC;Viswanathan, S;Nouws, HP;Delerue-Matos, C;González-García, MB;
Journal
Talanta
Title
Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD
Year
2014
Author
Marques, RC;Viswanathan, S;Nouws, HP;Delerue-Matos, C;González-García, MB;
Journal
Talanta
Title
Phage display as a tool for development of novel therapeutics for breast cancer
Year
2014
Author
Cabral, MJCD;
Journal
Thesis
Title
Phage display as a tool for development of novel therapeutics for breast cancer
Year
2014
Author
Cabral, MJCD;
Journal
Thesis
Title
Increased expression of EHF via gene amplification contributes to the activation of HER family signaling and associates with poor survival in gastric cancer
Year
2016
Author
Shi, J;Qu, Y;Li, X;Sui, F;Yao, D;Yang, Q;Shi, B;Ji, M;Hou, P;
Journal
Cell Death Dis
Title
para-Sulfonatocalix[4]arene stabilized gold nanoparticles multilayers interfaced to electrodes through host-guest interaction for sensitive ErbB2 detection.
Year
2017
Author
Wang, X;Du, D;Dong, H;Song, S;Koh, K;Chen, H;
Journal
Biosens Bioelectron
Title
para-Sulfonatocalix[4]arene stabilized gold nanoparticles multilayers interfaced to electrodes through host-guest interaction for sensitive ErbB2 detection.
Year
2017
Author
Wang, X;Du, D;Dong, H;Song, S;Koh, K;Chen, H;
Journal
Biosens Bioelectron
Title
Ultra-low fouling methylimidazolium modified surfaces for the detection of HER2 in breast cancer cell lysates
Year
2017
Author
Aubé, A;Campbell, S;Schmitzer, AR;Claing, A;Masson, JF;
Journal
Analyst
Title
Ultra-low fouling methylimidazolium modified surfaces for the detection of HER2 in breast cancer cell lysates
Year
2017
Author
Aubé, A;Campbell, S;Schmitzer, AR;Claing, A;Masson, JF;
Journal
Analyst
Title
Ultra-low fouling methylimidazolium modified surfaces for the detection of HER2 in breast cancer cell lysates
Year
2017
Author
Aubé, A;Campbell, S;Schmitzer, AR;Claing, A;Masson, JF;
Journal
Analyst
Title
Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
Year
2017
Author
Marques, RC;Costa-Rama, E;Viswanathan, S;Nouws, HP;Costa-Garcıa, A;Delerue-Matos, C;Gonzalez-Garcıa, MB;
Journal
Sensors and Actuators B: Chemical
Title
基于光波导微环谐振器的 lgG 非标记检测微流体分析系统
Year
2017
Author
郭盈盈;吴尚犬;金里;王皖君;张青川;
Journal
分析化学
Title
基于光波导微环谐振器的 lgG 非标记检测微流体分析系统
Year
2017
Author
郭盈盈;吴尚犬;金里;王皖君;张青川;
Journal
分析化学
Title
Construction of humanized anti-HER2 single-chain variable fragments (husFvs) and achievement of potent tumor suppression with the reconstituted husFv-Fdt-tBid immunoapoptotin
Year
2018
Author
Ou-Yang, Q;Yan, B;Li, A;Hu, ZS;Feng, JN;Lun, XX;Zhang, MM;Zhang, MD;Wu, KC;Xue, FF;Yang, AG;Zhao, J;
Journal
Biomaterials
Title
ELISA assay employing epitope-specific monoclonal antibodies to quantify circulating HER2 with potential application in monitoring cancer patients undergoing therapy with trastuzumab
Year
2020
Author
Agnolon, V;Contato, A;Meneghello, A;Tagliabue, E;Toffoli, G;Gion, M;Polo, F;Fabricio, ASC;
Journal
Sci Rep