EphB2 Protein Overview: Sequence, Structure, Function and Protein Interaction

EphB2 Protein Overview

Chan and Watt (1991) cloned partial sequences of the EEK (EPHA8; 176945) and ERK genes encoding members of the EPH subclass of receptor protein-tyrosine kinases. Northern blot analysis of rat RNA showed that DNA encoding human ERK hybridized to transcripts most abundantly in lung. By screening a human fetal brain cDNA expression library using a monoclonal antiphosphotyrosine antibody and by 5-prime RACE (rapid amplification of cDNA ends) procedures, Ikegaki et al. (1995) isolated overlapping cDNAs encoding a receptor-type tyrosine kinase belonging to the EPH family and designated the gene DRT (for developmentally regulated EPH-related tyrosine kinase). The DRT gene is expressed in transcripts of 3 different sizes (4, 5, and 11 kb). The DRT transcripts are expressed in human brain and several other tissues, including heart, lung, kidney, placenta, pancreas, liver, and skeletal muscle, but the 11-kb DRT transcript is preferentially expressed in fetal brain. Steady-state levels of DRT mRNA in several tissues, including brain, heart, lung, and kidney, are greater in the midterm fetus than those in the adult. Ikegaki et al. (1995) showed that a large number of tumor cell lines derived from neuroectoderm express DRT transcripts. The authors speculated that DRT may play a part in human neurogenesis. Northern blot analysis by Fox et al. (1995) revealed that HEK5 is expressed as transcripts of several sizes in a variety of human tissues, with the highest level of expression in placenta. Saito et al. (1995) demonstrated from the cDNA sequence that the ERK protein has a highly hydrophobic portion upstream of the putative tyrosine kinase domain, suggesting that it possesses a receptor-like membrane-spanning structure. Using immunohistochemical analysis of the developing mouse hindbrain, Cowan et al. (2000) detected Ephb2 expression in the midline, including the floor plate and the ependymal layer.

EphB2 protein family

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

EphB2 protein name

Recommended name
Ephrin type-B receptor 2
Hek5, Tyro5
Alternative name
Developmentally-regulated Eph-related tyrosine kinase ELK-related tyrosine kinase EPH tyrosine kinase 3 EPH-like kinase 5 EK5 hEK5 Renal carcinoma antigen NY-REN-47 Tyrosine-protein kinase TYRO5 Tyrosine-protein kinase receptor EPH-3

EphB2 Gene family protein

EphB2 Protein Sequence

Species Human EphB2 protein
Length 1055
Mass (Da) 117493
Sequence Human EphB2 protein sequence
Species Mouse EphB2 protein
Length 986
Mass (Da) 109899
Sequence Mouse EphB2 protein sequence
Mass (Da)

EphB2 Protein Molecular Weight & PI

Ephrin type-B receptor 2 precursor (EC (Developmentally-regulated Eph-related tyrosine kinase) (ELK-related tyrosine kinase) (EPH tyrosine kinase 3) (EPH-like kinase 5) (EK5) (hEK5) (Renal carcinoma antigen NY-REN-47) (Tyrosine-protein kinase TYRO5) (Tyrosine-protein kinase receptor EPH-3) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 19-1055 Ephrin type-B receptor 2.

Molecular weight (Da)


Theoretical pI


EphB2 Protein Structure

1998-12-20   Released:  1999-02-16
Deposition Author(s)
Thanos, C.D., Goodwill, K.E., Bowie, J.U.
Homo sapiens
Expression System
Escherichia coli BL21
Experimental Data Snapshot
1.9500 Å
R-Value Free
R-Value Work
1B4F From PDB

Human EphB2 protein Secondary structure

EphB2 Protein Interaction

Recombinant EphB2 Protein Feature

EphB2 Protein, Human, Recombinant (His & hFc Tag)

High Purity
> 90 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to bind recombinant human EphrinB2 in a functional ELISA.

Recombinant EphB2 protein citations

A novel autoantibody against ephrin type B receptor 2 in acute necrotizing encephalopathy
Shirai, T;Fujii, H;Ono, M;Watanabe, R;Shirota, Y;Saito, S;Ishii, T;Nose, M;Harigae, H;
J Neuroinflammation
EphrinB/EphB signaling contributes to spinal nociceptive processing via calpain‑1 and caspase‑3
Yang, M;Chen, W;Zhang, Y;Yang, R;Wang, Y;Yuan, H;
Mol Med Rep
Drug Conjugates for Targeting Eph Receptors in Glioblastoma
Sharma, P;Roberts, C;Herpai, D;Fokt, I;Priebe, W;Debinski, W;

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