Clusterin Antibody Guideline: Preparation, Dilution and Applications

Clusterin Antibody Overview

There are 15 Clusterin antibodies which are validated in multiple tissues with various applications, including WB, ELISA, FCM, ICC/IF, IHC-P, IP. There are 2 Clusterin antibody for WB, 8 Clusterin antibody for ELISA, 5 Clusterin antibody for FCM, 3 Clusterin antibody for ICC/IF, 1 Clusterin antibody for IHC-P, 1 Clusterin antibody for IP. Among all these Clusterin antibodies, there are 3 anti-Clusterin mouse monoclonal antibodies , 5 anti-Clusterin rabbit monoclonal antibodies , 7 anti-Clusterin rabbit polyclonal antibodies . All the Clusterin anbodies are produced in house and all are in stock. Clusterin antibody customerized service is available.

Clusterin Antibody ELISA

Clusterin antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin / Apolipoprotein J / Apo-J; Catalog#11297-H08H; NP_001822.2; Met 1-Glu 501).

Clusterin antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

Clusterin Antibody western blot

Clusterin Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin / Apolipoprotein J / Apo-J; Catalog#11297-H08H; NP_001822.2; Met 1-Glu 501). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Clusterin Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Clusterin Antibody flow cytometry

Clusterin Antibody immunohistochemistry

Clusterin Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J / CLU (rh Clusterin / Apolipoprotein J / Apo-J / CLU; Catalog#11297-H08H; NP_001822.2; Met1-Glu501).

Clusterin Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

Clusterin Antibody immunoprecipitation

Clusterin Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin; Catalog#11297-H08H; NP_001822.2; Met 1-Glu 501). Clusterin specific IgG was purified by Human Clusterin affinity chromatography.

Clusterin Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

Clusterin Antibody ICC / IF

Clusterin Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin / Apolipoprotein J / Apo-J; Catalog#11297-H08H; NP_001822.2; Met1-Glu501).

Clusterin Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

Clusterin antibody citations

Title
Clusterin and neuropilin-2 as potential biomarkers of tumor progression in benzo[a]pyrene-transformed 16HBE cells xenografted nude mouse model
Year
2017
Author
Wang, Y;Jia, Y;Yan, L;Fu, J;Hao, M;Chen, W;Yao, B;Zhao, P;Zhou, Z;
Journal
Chem. Biol. Interact.
Title
Colorimetric Paper-Based Immunosensor for Simultaneous Determination of Fetuin B and Clusterin toward Early Alzheimer's Diagnosis
Year
2019
Author
Brazaca, LC;Moreto, JR;Martín, A;Tehrani, F;Wang, J;Zucolotto, V;
Journal
ACS Nano
Title
Clusterin role in hepatocellular carcinoma patients treated with oxaliplatin Short title: CLU in HCC
Year
2020
Author
Wang, X;Liu, Y;Qin, Q;Zheng, T;
Journal
portlandpress.com
Title
Clusterin role in hepatocellular carcinoma patients treated with oxaliplatin
Year
2020
Author
Wang, X;Liu, Y;Qin, Q;Zheng, T;
Journal
Biosci. Rep.