CD73 Protein Overview: Sequence, Structure, Function and Protein Interaction

CD73 Protein Overview

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see 102700, 300755). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.

CD73 protein family

Belongs to the 5'-nucleotidase family.

CD73 protein name

Recommended name
Short name
CALJA, CD73, eN, eNT
Alternative name
Ecto-5'-nucleotidase CD_antigen: CD73

CD73 Protein Sequence

Species Human CD73 protein
Length 574
Mass (Da) 63368
Sequence Human CD73 protein sequence
Species Mouse CD73 protein
Length 576
Mass (Da) 63864
Sequence Mouse CD73 protein sequence
Species Rat CD73 protein
Length 576
Mass (Da) 63969
Sequence Rat CD73 protein sequence

CD73 Protein Molecular Weight & PI

5'-nucleotidase precursor (EC (5'-NT) (Ecto-5'-nucleotidase) (CD73 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 27-549 5'-nucleotidase.

Molecular weight (Da)


Theoretical pI


CD73 Protein Structure

Crystal Structure of a Truncated Soluble form of Human CD73 with Ecto-5'-Nucleotidase activity
2012-09-11   Released:  2012-10-03
Deposition Author(s)
Heuts, D.P., Weissenborn, M.J., Olkhov, R.V., Shaw, A.M., Levy, C.W., Scrutton, N.S.
Homo sapiens
Experimental Data Snapshot
2.2000 Å
R-Value Free
R-Value Work
4H1S From PDB

Human CD73 protein Secondary structure

CD73 Protein Interaction

Recombinant CD73 Protein Feature

CD73 Protein, Cynomolgus, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is >20,000 pmol/min/µg.

Recombinant CD73 protein citations

Adenosine Receptor Agonists And CD73 Effects On Avm Cell Calcification Markers
Koliopoulos, T;

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