ATP citrate lyase/ACLY Protein Overview: Sequence, Structure, Function and Protein Interaction

ATP citrate lyase/ACLY Protein Overview

ATP citrate lyase/ACLY reagents

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.

ATP citrate lyase/ACLY protein family

In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.

ATP citrate lyase/ACLY protein name

Recommended name
ATP-citrate synthase
Aliases
ACL, ATP citrate synthase, ATPCL, CLATP
Alternative name
ATP-citrate (pro-S-)-lyase ACL Citrate cleavage enzyme

ATP citrate lyase/ACLY Protein Sequence

Species Human ATP citrate lyase/ACLY protein
Length 1101
Mass (Da) 120839
Sequence Human ATP citrate lyase/ACLY protein sequence
Species Mouse ATP citrate lyase/ACLY protein
Length 1091
Mass (Da) 119728
Sequence Mouse ATP citrate lyase/ACLY protein sequence
Species Rat ATP citrate lyase/ACLY protein
Length 1100
Mass (Da) 120636
Sequence Rat ATP citrate lyase/ACLY protein sequence

ATP citrate lyase/ACLY Protein Molecular Weight & PI

ATP-citrate synthase (EC 2.3.3.8) (ATP-citrate (pro-S-)-lyase) (ACL) (Citrate cleavage enzyme)Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-1101 ATP-citrate synthase.

Molecular weight (Da)

120839.23

Theoretical pI

6.95

ATP citrate lyase/ACLY Protein Structure

C20S, C293G Mutant N-terminal Human ATP Citrate Lyase Bound to 4R-Hydroxycitrate
Deposited
2016-09-20   Released:  2017-08-09
Deposition Author(s)
Hu, J., Fraser, M.E.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.2500 Å
R-Value Free
0.247
R-Value Work
0.188
5TE1 From PDB

Human ATP citrate lyase/ACLY protein Secondary structure

ATP citrate lyase/ACLY Protein Interaction

Recombinant ATP citrate lyase/ACLY Protein Feature

ATP citrate lyase/ACLY Protein, Human, Recombinant (His Tag)

High Purity
> 85 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method

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