ACP5 Protein Overview: Sequence, Structure, Function and Protein Interaction

ACP5 Protein Overview

ACP5 reagents

Ketcham et al. (1988) cloned a full-length cDNA for type 5 tartrate-resistant acid phosphatase from human placenta and found that it contained an open reading frame of 969 basepairs (corresponding to a protein of 323 amino acids), a putative signal sequence of 19 amino acids, and 2 potential glycosylation sites. The deduced amino acid sequence of the human isozyme is 85% identical to the amino acid sequence of porcine uteroferrin and 82% identical to the corresponding regions of a partial amino acid sequence of the bovine spleen enzyme. The type 5 isozyme of acid phosphatase is the most basic of the acid phosphatases and is the only form insensitive to inhibition by L(+)-tartrate. Normally, it is detected as a minor intracellular component of spleen, lung, liver, and bone. High levels of acid phosphatase-5 are found within the spleen and monocytes of patients with Gaucher disease (230800). The type 5 isozyme is an iron-containing glycoprotein with a molecular mass of approximately 34 kD.Lord et al. (1990) also isolated a clone for ACP5, which is found principally in resident tissue macrophages. They found that an ACP5 cDNA clone contained an open reading frame of 975 bp, encoded a protein of 325 amino acids, including a single peptide of 19 residues and 2 potential sites for N-glycosylation.

ACP5 protein family

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

ACP5 protein name

Recommended name
Tartrate-resistant acid phosphatase type 5
Short name
TR-AP
Aliases
HPAP, human purple acid phosphatase, tartrate-resistant acid phosphatase, TRAP
Alternative name
Tartrate-resistant acid ATPase TrATPase Type 5 acid phosphatase

ACP5 Gene family protein

ACP5 Protein Sequence

Species Human ACP5 protein
Length 325
Mass (Da) 36599
Sequence Human ACP5 protein sequence
Species Mouse ACP5 protein
Length 327
Mass (Da) 36807
Sequence Mouse ACP5 protein sequence
Species Rat ACP5 protein
Length 327
Mass (Da) 36726
Sequence Rat ACP5 protein sequence

ACP5 Protein Molecular Weight & PI

Tartrate-resistant acid phosphatase type 5 precursor (EC 3.1.3.2) (TR-AP) (Tartrate-resistant acid ATPase) (TrATPase) (Type 5 acid phosphatase)Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 22-325 Tartrate-resistant acid phosphatase type

Molecular weight (Da)

34331

Theoretical pI

9.11

ACP5 Protein Structure

Recombinant Human Purple Acid Phosphatase expressed in Pichia Pastoris
Deposited
2004-10-28   Released:  2005-07-06
Deposition Author(s)
Duff, A.P., Langley, D.B., Han, R., Averill, B.A., Freeman, H.C., Guss, J.M.
Organism(s)
Homo sapiens
Expression System
Komagataella pastoris
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.2200 Å
R-Value Free
0.198
R-Value Work
0.138
1WAR From PDB

Human ACP5 protein Secondary structure

ACP5 Protein Interaction

Recombinant ACP5 Protein Feature

ACP5 Protein, Human, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method

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