ACOT1 Protein Overview: Sequence, Structure, Function and Protein Interaction

ACOT1 Protein Overview

ACOT1 reagents

By searching a database for orthologs of mouse Acot genes, Hunt et al. (2006) identified human ACOT1. The deduced 421-amino acid protein has a putative catalytic site and a possible C-terminal peroxisome-targeting signal. ACOT1 shares 98.6% amino acid identity with the corresponding 421 amino acids of ACOT2 (609972); however, ACOT2 has a 62-amino acid mitochondrial targeting signal at its N-terminal end that is absent in ACOT1. Fluorescence-tagged ACOT1 showed a diffuse pattern of expression in transfected human skin fibroblasts. Brocker et al. (2010) reviewed the ACOT gene family. They stated that ACOT1 contains an N-terminal acyl-CoA thioester hydrolase domain and a C-terminal esterase-lipase domain. The N-terminal domain does not participate directly in catalysis but appears to regulate enzyme activity. The C-terminal domain, also known as an alpha/beta-hydrolase fold, contains all residues required for catalysis. ACOT2 and ACOT4 (614314) have the same protein structure.

ACOT1 protein family

Belongs to the C/M/P thioester hydrolase family.

ACOT1 protein name

Recommended name
Acyl-coenzyme A thioesterase 1

ACOT1 Gene family protein

Acyl-CoA thioesterases
Approved symbol Common name
ACOT13 ACOT13/THEM2 protein, recombinant

ACOT1 Protein Molecular Weight & PI

The parameters have been computed for the following feature

FT CHAIN 2-421 Acyl-coenzyme A thioesterase 1.

Molecular weight (Da)


Theoretical pI


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