Acetylcholinesterase Protein Overview: Sequence, Structure, Function and Protein Interaction

Acetylcholinesterase Protein Overview

Acetylcholinesterase reagents

Acetylcholinesterase (ACHE; EC 3.1.1.7) controls synaptic and neurohumoral cholinergic activity by hydrolyzing the neurotransmitter acetylcholine. ACHE function relies on precise regulation of its expression and localization. In particular, alternative splicing of the 3-prime region of ACHE results in ACHE isoforms with distinct C-terminal peptides that determine posttranslational maturation and oligomeric assembly (summary by Xie et al., 2010).

Acetylcholinesterase protein family

Belongs to the type-B carboxylesterase/lipase family.

Acetylcholinesterase protein name

Recommended name
Acetylcholinesterase
Short name
AChE
Aliases
Yt blood group

Acetylcholinesterase Protein Sequence

Species Human Acetylcholinesterase protein
Length 614
Mass (Da) 67796
Sequence Human Acetylcholinesterase protein sequence
Species Mouse Acetylcholinesterase protein
Length 614
Mass (Da) 68169
Sequence Mouse Acetylcholinesterase protein sequence
Species Rat Acetylcholinesterase protein
Length 614
Mass (Da) 68196
Sequence Rat Acetylcholinesterase protein sequence

Acetylcholinesterase Protein Molecular Weight & PI

Acetylcholinesterase precursor (EC 3.1.1.7) (AChE)Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 32-614 Acetylcholinesterase.

Molecular weight (Da)

64576.07

Theoretical pI

5.75

Acetylcholinesterase Protein Structure

Structure of the tetramerization domain of acetylcholinesterase: four-fold interaction of a WWW motif with a left-handed polyproline helix
Deposited
2004-05-20   Released:  2005-01-10
Deposition Author(s)
Dvir, H., Harel, M., Bon, S., Liu, W.-Q., Vidal, M., Garbay, C., Sussman, J.L., Massoulie, J., Silman, I.
Organism(s)
Homo sapiens
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.3500 Å
R-Value Free
0.259
R-Value Work
0.247
1VZJ From PDB

Human Acetylcholinesterase protein Secondary structure

Acetylcholinesterase Protein Interaction

Recombinant Acetylcholinesterase Protein Feature

Acetylcholinesterase Protein, Mouse, Recombinant (His Tag)

High Purity
> 97 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to cleave Acetylthiocholine. The specific activity is > 250 nmols/min/μg.

Recombinant Acetylcholinesterase protein citations

Title
Mouse acetylcholinesterase enhances neurite outgrowth of rat R28 cells through interaction with laminin-1
Year
2012
Author
Sperling, LE;Klaczinski, J;Schütz, C;Rudolph, L;Layer, PG;
Journal
PLoS ONE

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