ACAP1/CENTB1 Protein Overview: Sequence, Structure, Function and Protein Interaction

ACAP1/CENTB1 Protein Overview

ACAP1/CENTB1 reagents

By sequencing clones obtained from a size-fractionated myeloid cell line cDNA library, Nomura et al. (1994) cloned CENTB1, which they designated KIAA0050. The deduced protein contains 740 amino acids and shares 52% sequence identity with CENTB2 (607766). Northern blot analysis revealed highest expression in spleen, thymus, and peripheral blood leukocytes, intermediate expression in lung, testis, and small intestine, and weak expression in prostate, ovary, and colon. No expression was detected in heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas, and no expression was detected in HeLa cells. Jackson et al. (2000) further characterized CENTB1, which they designated ACAP1. They identified a coiled-coil region at its N terminus, followed by an X-box domain, a pleckstrin homology (PH) domain, an ARF-GAP domain (see ARF6; 600464), and C-terminal ankyrin repeats. ACAP1 shares significant similarity with CENTB2, which the authors called ACAP2, and 95% identity with mouse Acap1. It also shares similarity with ASAP1 (605953) and PAP (603817) in the ARF-GAP domain and in the overall domain structure. Jackson et al. (2000) also identified homologous sequences in C. elegans, A. thaliana, and D. melanogaster. By semiquantitative PCR, they detected ACAP1 mRNA expressed at highest levels in spleen and lung and at intermediate levels in heart, kidney, liver, and pancreas. Little or no expression was detected in testis and brain. PCR analysis showed 2 additional minor products in spleen, lung, and pancreas. Western blot analysis detected ACAP1 in all cell lines examined except monocytes.

ACAP1/CENTB1 protein name

Recommended name
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
Aliases
KIAA0050

ACAP1/CENTB1 Protein Molecular Weight & PI

The parameters have been computed for the following feature

FT CHAIN 1-740 Arf-GAP with coiled-coil, ANK repeat and

Molecular weight (Da)

81535.87

Theoretical pI

7.60

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