SARS-CoV-2 Spike & Nucleocapsid Mutant Protein

A distinct strain of SARS-CoV-2 emerges in the UK recently, reportedly accounting for over 60% new infections in the London area. CDC also names the variant "SARS-CoV-2 VOC (variant of concern) 202012/01", highlighting its clinical significance.

The newly identified lineage B.1.1.7 carries an unusually high number of genetic mutations, resulting in an unprecedented 14 AA replacement. Eight of these mutations occur in the spike protein and the following three are particularly worrisome.
-- N501Y, which reportedly leads to an increased binding affinity to human and murine ACE2 receptors
-- 69-70del, which arose from the outbreak linked to farmed minks
-- P681H, which is closed to the furin cleavage site.

Mutation N501Y is also found in a new South Africa variant (known as 20C/501Y.V2 or B.1.351 lineage) along with other two mutations, K417N and E484K, happened in Spike receptor binding domain.

It's also important to monitor the B.1.1.7 strain together with the previously identified high frequency variants, such as
-- D614G, which may cause a moderately increased transmissibility
-- N439K, which may be an "escape mutant" for some neutralizing antibodies
-- Y453F, which may be an "escape mutant" for some neutralizing antibodies.

Sino Biological has produced a collection of recombinant antigens for these variants. The reagents can be used to evaluate the efficacy of the antibodies and vaccination.

Resource for Studying the U.K. Variant

Catalogue  Order  Antigen  Mutation 
40592-V08H82 Order  RBD  N501Y 
40591-V08H7 Order  S1  HV69-70 deletion, N501Y, D614G 
40591-V08H12 Order  S1  H69del, V70del, Y144del, N501Y, A570D, D614G, P681H 
40588-V07E7  Pre-Order  D3L, R203K, G204R, S235F 
40588-V07E8  Pre-Order  D3L, S235F 
40589-V08B6  Under Development  S-ECD H69del, V70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H 
Recombinant Spike N501Y Mutant
Cat#: 40592-V08H82
Activity: Bind with ACE2

SARS-CoV-2 Spike RBD Mutant

  • RBD His Tag
    P337S
    40592-V08H62
  • RBD His Tag
    F338L
    40592-V08H26
  • RBD His Tag
    V341I
    40592-V08H11
  • RBD His Tag
    F342L
    40592-V08H6
  • RBD His Tag
    A344S
    40592-V08H37
  • RBD His Tag
    A348S
    40592-V08H25
  • RBD His Tag
    A352S
    40592-V08H58
  • RBD His Tag
    N354D
    40592-V08H2
  • RBD His Tag
    S359N
    40592-V08H32
  • RBD mFc Tag
    V367F
    40592-V05H1
  • RBD His Tag
    V367F
    40592-V08H1
  • RBD His Tag
    N370S
    40592-V08H43
  • RBD His Tag
    A372S
    40592-V08H19
  • RBD His Tag
    A372T
    40592-V08H36
  • RBD His Tag
    F377L
    40592-V08H27
  • RBD His Tag
    K378N
    40592-V08H42
  • RBD His Tag
    K378R
    40592-V08H33
  • RBD His Tag
    P384L
    40592-V08H24
  • RBD His Tag
    T385A
    40592-V08H47
  • RBD His Tag
    T393P
    40592-V08H48
  • RBD His Tag
    V395I
    40592-V08H49
  • RBD His Tag
    D405V, Q414A
    40592-V08H22
  • RBD His Tag
    E406Q
    40592-V08H40
  • RBD His Tag
    R408I
    40592-V08H10
  • RBD His Tag
    Q409E
    40592-V08H34
  • RBD His Tag
    Q414E
    40592-V08H23
  • RBD His Tag
    Q414R
    40592-V08H44
  • RBD His Tag
    K417N
    40592-V08H59
  • RBD His Tag
    A435S
    40592-V08H4
  • RBD His Tag
    W436R
    40592-V08H9
  • RBD His Tag
    N439K
    40592-V08H14
  • RBD His Tag
    N440K
    40592-V08H55
  • RBD His Tag
    K444R
    40592-V08H54
  • RBD His Tag
    V445F
    40592-V08H79
  • RBD His Tag
    G446S
    40592-V08H76
  • RBD His Tag
    G446V
    40592-V08H51
  • RBD His Tag
    L452R
    40592-V08H28
  • RBD His Tag
    Y453F
    40592-V08H80
  • RBD His Tag
    L455F
    40592-V08H68
  • RBD His Tag
    F456E
    40592-V08H73
  • RBD His Tag
    F456L
    40592-V08H71
  • RBD His Tag
    K458Q
    40592-V08H69
  • RBD His Tag
    K458R
    40592-V08H7
  • RBD His Tag
    E471Q
    40592-V08H56
  • RBD His Tag
    I472V
    40592-V08H35
  • RBD His Tag
    G476S
    40592-V08H8
  • RBD His Tag
    S477I
    40592-V08H45
  • RBD His Tag
    S477N
    40592-V08H46
  • RBD His Tag
    S477R
    40592-V08H64
  • RBD His Tag
    T478I
    40592-V08H30
  • RBD His Tag
    P479S
    40592-V08H57
  • RBD His Tag
    N481D
    40592-V08H70
  • RBD His Tag
    G482S
    40592-V08H53
  • RBD His Tag
    V483A
    40592-V08H5
  • RBD His Tag
    V483I
    40592-V08H31
  • RBD His Tag
    E484K
    40592-V08H84
  • RBD His Tag
    E484Q
    40592-V08H81
  • RBD His Tag
    G485S
    40592-V08H52
  • RBD His Tag
    F486S
    40592-V08H74
  • RBD His Tag
    N487R
    40592-V08H75
  • RBD His Tag
    F490L
    40592-V08H83
  • RBD His Tag
    F490S
    40592-V08H41
  • RBD His Tag
    S494P
    40592-V08H18
  • RBD His Tag
    P499R
    40592-V08H78
  • RBD His Tag
    N501Y
    40592-V08H82
  • RBD His Tag
    V503F
    40592-V08H15
  • RBD His Tag
    Y505C
    40592-V08H72
  • RBD His Tag
    Y508H
    40592-V08H12
  • RBD His Tag
    A520S
    40592-V08H20
  • RBD His Tag
    A520V
    40592-V08H39
  • RBD His Tag
    P521R
    40592-V08H63
  • RBD His Tag
    P521S
    40592-V08H29
  • RBD His Tag
    A522S
    40592-V08H21
  • RBD His Tag
    A522V
    40592-V08H16

SARS-CoV-2 Spike S1 Mutant

SARS-CoV-2 Spike S1+S2 ECD Mutant

Recombinant Antibody Production