200+ SARS-CoV-2 Variants

Several new variants of SARS-CoV-2 virus have emerged in recent months. The U.K. variant B.1.1.7, the Brazil variant P.1, the South Africa variant B.1.351, and the most recent Indian variant B.1.617 are particular concerning because of their high prevalence. A subset of the mutations identified in the RBD domain of the spike protein occurs in more than one strain, These convergent mutations are of high interest because they may be the cause of the increased transmissibility. Sino Biological has launched RBD and Spike proteins of these variants.

The mutations in these strains also occur in the nucleocapsid protein, which is commonly used as the biomarker in rapid antigen tests. It’s critical to assess whether the current commercial antigen tests can detect the mutated N proteins with the same sensitivity and specificity as their WT counterpart.

By monitoring the evolution of SARS-CoV-2, WHO has published these Variants of Concern (VOCs) to accelerate the globe to make quick response to the ongoing COVID-19 pandemic.
Characteristics of VOCs can be:
• Increase in transmissibility or detrimental change in COVID-19 epidemiology
• Increase in virulence or change in clinical disease presentation
• Decrease in effectiveness of public health and social measures or available diagnostics, vaccines, therapeutics

Sino Biological has developed a panel of recombinant RBD/Spike and nucleocapsid protein variants which can be used to evaluate the efficacy of the antibodies and vaccination.

Recombinant SARS-CoV-2 Variants

Prefusion Trimeric Spike Variant

SARS-CoV-2 Variant Detection Antibodies

Mu (B.1.621)

• Variants in S Protein

Label Lineage Catalogue Antigen Mutations
Mu B.1.621 40589-V08H20 (pre-order) S1+S2 ECD T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H
Mu B.1.621 40591-V08H38 (pre-order) S1 T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H
Mu B.1.621 40592-V08H118 (pre-order) RBD R346K, E484K, N501Y
Mu B.1.621 40592-V02H6 (pre-order) RBD R346K, E484K, N501Y

Lambda (C.37)

Label Lineage Catalogue Antigen Mutations
Lambda C.37 40588-V07E31 N P13L, R203K, G204R, G214C
Lambda C.37 40592-V08H113 RBD L452Q, F490S
Lambda C.37 40589-V08B23 (pre-order) S1+S2 ECD G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G, T859N
Lambda C.37 40589-V08B24 (pre-order) S1+S2 ECD G75V, T76I, R246del, S247del, Y248del, L249del, T250del, P251del, G252del, D253N, L452Q, F490S, D614G, T859N
Lambda C.37 40591-V08H31 S1 G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G
Lambda C.37 40592-V49H6-B RBD L452Q, F490S

Delta/Delta plus (AY.1, AY.2, AY.3)

Label Lineage Catalogue Antigen Mutations
Delta/Delta plus AY.1/AY.3 40588-V07E32 N D63G, R203M, G215C, D377Y
Delta/Delta plus AY.1/AY.2 40592-V08H115 RBD K417N, L452R, T478K
Delta/Delta plus AY.1/AY.2 40592-V49H5-B RBD K417N, L452R, T478K
Delta/Delta plus AY.1 40589-V08B25 (pre-order) S1+S2 ECD T19R, G142D, W258L, K417N, L452R, T478KD614G, D950N, P681R
Delta/Delta plus AY.2 40589-V08B26 (pre-order) S1+S2 ECD T19R, V70F, E156G, del157/158, A222V, K417N, L452R, T478K, D614G, P681R, D950N
Delta/Delta plus AY.3 40589-V08B27 (pre-order) S1+S2 ECD T19R, E156G, del157/158, L452R, T478K, D614G, P681R, D950N

Delta (B.1.617.2)

• Variants in S Protein

• Variants in N Protein

• Recombinant Delta (B.1.617.2) Variants

Label Lineage Catalogue Antigen Mutations
Delta B.1.617.2 40588-V07E29 N D63G, R203M, D377Y
Delta B.1.617.2 40589-V49B4-B S1+S2 ECD 157-158deletion, T19R, G142D, E156G, L452R, T478K, D614G, P681R, D950N
Delta B.1.617.2 40589-V08B16 S1+S2 ECD T19R, G142D, del157/158, L452R, T478K, D614G, P681R, D950N
Delta B.1.617.2 40591-V08H23 S1 T19R, G142D, E156G, del157/158, L452R, T478K, D614G, P681R
Delta B.1.617.2 40591-V49H2-B S1 (157-158)deletion, T19R, G142D, E156G, L452R, T478K, D614G, P681R
Delta B.1.617.2 40592-V02H3 RBD L452R, T478K
Delta B.1.617.2 40592-V08H90 RBD L452R, T478K
Delta B.1.617.2 40592-V08H91 RBD T478K
Delta B.1.617.2 40592-V49H1-B RBD L452R, T478K

Alpha/Beta (B.1.1.7/ B.1.351/ A.2.2)

Label Lineage Catalogue Antigen Mutations
Alpha/Beta B.1.1.7/ B.1.351/ A.2.2 40588-V07E2 N P13L

Alpha/Beta/Gamma (B.1.1.7/ B.1.351/ P.1)

Label Lineage Catalogue Antigen Mutations
Alpha/Beta/Gamma B.1.1.7/ B.1.351/ P.1 40592-V31H1 RBD N501Y

Alpha (B.1.1.7)

• Variants in S Protein

• Variants in N Protein

• Recombinant Alpha (B.1.1.7) Variants

Label Lineage Catalogue Antigen Mutations
Alpha B.1.1.7 40588-V07E1 N R203K, G204R
Alpha B.1.1.7 40588-V07E17 N E378Q
Alpha B.1.1.7 40588-V07E4 N I292T
Alpha B.1.1.7 40588-V07E7 N D3L, R203K, G204R, S235F
Alpha B.1.1.7 40588-V07E8 N D3L, S235F
Alpha B.1.1.7/ B.1.237 40588-V07E3 N S194L
Alpha B.1.1.7 40589-V08B6 S1+S2 ECD H69del, V70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
Alpha B.1.1.7 40589-V49B-B S1+S2 ECD H69del, V70del, Y145del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
Alpha B.1.1.7 40591-V08H12 S1 H69del, V70del, Y144del, N501Y, A570D, D614G, P681H
Alpha B.1.1.7 40591-V08H12-B S1-Biotinylated H69del, V70del, Y144del, N501Y, A570D, D614G, P681H
Alpha B.1.1.7 40591-V08H7 S1 HV69-70deletion, N501Y, D614G
Alpha B.1.1.7 40591-V49H4-B S1 (HV69-70, Y145)deletion, N501Y, A570D, D614G, P681H
Alpha B.1.1.7 40592-V02H1 RBD N501Y
Alpha B.1.1.7 40592-V08H18 RBD S494P
Alpha B.1.1.7 40592-V08H82 RBD N501Y
Alpha B.1.1.7 40592-V08H82-B RBD N501Y
Alpha B.1.1.7 40592-V49H2-B RBD N501Y

Beta (B.1.351)

• Variants in S Protein

• Variants in N Protein

• Recombinant Beta (B.1.351) Variants

Label Lineage Catalogue Antigen Mutations
Beta B.1.351/B.1.351.2/
B.1.351.3/B.1.427/B.1.429
40588-V07E9 N T205I
Beta B.1.351 40589-V08B11 S1+S2 ECD D80A, L242del, A243del, L244del, R246I, E484K, K417N, N501Y, D614G, A701V
Beta B.1.351/B.1.351.2/B.1.351.3 40589-V08B7 S1+S2 ECD L18F, D80A, D215G, LAL242-244deletion, R246I, K417N, E484K, N501Y, D614G, A701V
Beta B.1.351/B.1.351.2/B.1.351.3 40589-V08B9 S1+S2 ECD D80A, K417N, E484K, N501Y, D614G, A701V
Beta B.1.351 40589-V49B1-B S1+S2 ECD
Beta B.1.351 40589-V08B7-B S1+S2-Biotinylated L18F, D80A, D215G, LAL242-244deletion, R246I, K417N, E484K, N501Y, D614G, A701V
Beta B.1.351/B.1.351.2/B.1.351.3 40591-V08H13 S1 NTD L18F, D80A, D215G, LAL242-244deletion, R246I
Beta B.1.351 40591-V08H10-B S1 K417N, E484K, N501Y, D614G
Beta B.1.351/B.1.351.2/B.1.351.3 40591-V08H10 S1 K417N, E484K, N501Y, D614G
Beta B.1.351/B.1.351.2/B.1.351.3 40591-V08H15 S1 L18F, D80A, D215G, LAL242-244deletion, R246I, K417N, E484K, N501Y, D614G
Beta B.1.351/B.1.351.2/B.1.351.3 40591-V49H5-B S1 (LAL242-244)deletion, L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G
Beta B.1.351 40592-V08H59 RBD K417N
Beta B.1.351 40592-V08H59-B RBD K417N
Beta B.1.351 40592-V08H84 RBD E484K
Beta B.1.351 40592-V08H84-B RBD E484K
Beta B.1.351 40592-V08H85-B RBD K417N, E484K, N501Y
Beta B.1.351 40592-V31H2 RBD E484K
Beta B.1.351 40592-V31H6 RBD K417N
Beta B.1.351/ B.1.351.3 40592-V31H4 RBD K417N, E484K, N501Y
Beta B.1.351/B.1.351.2/B.1.351.3 40592-V02H4 RBD K417N, E484K, N501Y
Beta B.1.351/B.1.351.2/B.1.351.3 40592-V08H85 RBD K417N, E484K, N501Y
Beta B.1.351/B.1.351.2/B.1.351.3 40592-V49H3-B RBD K417N, E484K, N501Y

Gamma (P.1/ P.1.1/ P.1.2)

• Variants in S Protein

• Variants in N Protein

• Recombinant Gamma (P.1/ P.1.1/ P.1.2) Variants

Label Lineage Catalogue Antigen Mutations
Gamma P.1/ P.1.1/ P.1.2 40588-V07E11 N P80R
Gamma P.1/ P.1.1/ P.1.2 40589-V08B10 S1+S2 ECD L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
Gamma P.1/ P.1.1/ P.1.2 40589-V08B8 S1+S2 ECD L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I
Gamma P.1/ P.1.1/ P.1.2 40591-V08H14 S1 L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y
Gamma P.1/ P.1.1/ P.1.2 40591-V08H5 S1 T20N, D614G
Gamma P.1/ P.1.1/ P.1.2 40591-V49H6-B S1 L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y
Gamma P.1/ P.1.1/ P.1.2 40592-V02H5 RBD K417T, E484K, N501Y
Gamma P.1/ P.1.1/ P.1.2 40592-V08H86 RBD K417T, E484K, N501Y
Gamma P.1/ P.1.1/ P.1.2 40592-V31H5 RBD K417T, E484K, N501Y
Gamma P.1/ P.1.1/ P.1.2 40592-V31H7 RBD K417T
Gamma P.1/ P.1.1/ P.1.2 40592-V49H4-B RBD E484K, K417T, N501Y

Epsilon (B.1.427/429)

Label Lineage Catalogue Antigen Mutations
Epsilon B.1.427 40591-V08H27 S1 L452R, D614G
Epsilon B.1.429/B.1.427 40591-V08H17 S1 W152C, L452R, D614G

Eta (B.1.525)

Label Lineage Catalogue Antigen Mutations
Eta B.1.525 40588-V07E19 N A12G, T205I
Eta B.1.525 40589-V08B20 S1+S2 ECD Q52R, A67V, 69/70del, 144del, E484K, D614G, Q677H, F888L
Eta B.1.525 40591-V08H29 S1 Q52R, A67V, 69/70del, 144del, E484K, D614G, Q677H
Eta B.1.525 40591-V08H16 S1 Q677H

Iota (B.1.526)

Label Lineage Catalogue Antigen Mutations
Iota B.1.526 40588-V07E18 N P199L, M234I
Iota B.1.526 40592-V08H112 RBD S477N, E484K
Iota B.1.526 40589-V08B21 S1+S2 ECD L5F, T95I, D253G, S477N, E484K, D614G, A701V
Iota B.1.526 40591-V08H28 S1 L5F, T95I, D253G, S477N, E484K, D614G

Kappa (B.1.617.1)

• Variants in S Protein

• Variants in N Protein

• Recombinant Kappa (B.1.617.1) Variants

Label Lineage Catalogue Antigen Mutations
Kappa B.1.617.1 40589-V49B3-B S1+S2 ECD T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H
Kappa B.1.617.1 40589-V08B15 S1+S2 ECD T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H
Kappa B.1.617.1 40591-V49H1-B S1 T95I, G142D, E154K, L452R, E484Q, D614G, P681R
Kappa B.1.617.1 40591-V08H22 S1 T95I, G142D, E154K, L452R, E484Q, D614G, P681R
Kappa B.1.617/B.1.617.1/B.1.617.3 40592-V02H2 RBD L452R, E484Q
Kappa B.1.617/B.1.617.1/B.1.617.3 40592-V08H88 RBD L452R, E484Q
Kappa B.1.617/B.1.617.1/B.1.617.3 40592-V08H88-B RBD L452R, E484Q
Kappa B.1.617/B.1.617.1/B.1.617.3 40592-V49H-B RBD L452R, E484Q

B.1.617

• Variants in S Protein

• Variants in N Protein

• Recombinant B.1.617 Variants

Lineage Catalogue Antigen Mutations
B.1.617 40588-V07E16 N D377Y
B.1.617 40588-V07E20 N R203M, D377Y
B.1.617 40589-V08B12 S1+S2 ECD D614G, E154K, E484Q, E1072K, K1073R, L452R, P681R
B.1.617 40589-V08B13 S1+S2 ECD D614G, E154K, E484Q, G142D, H1101D, L452R, P681R, Q1071H
B.1.617 40589-V49B2-B S1+S2 ECD
B.1.617 40591-V08H19 S1 D614G, E154K, E484Q, L452R, P681R
B.1.617 40592-V08H28 RBD L452R
B.1.617 40592-V08H81 RBD E484Q

B.1.617.3

• Variants in S Protein

• Variants in N Protein

• Recombinant B.1.617.3 Variants

Lineage Catalogue Antigen Mutations
B.1.617.3 40588-V07E30 N P67S, R203M, D377Y
B.1.617.3 40589-V49B5-B S1+S2 ECD T19R, L452R, E484Q, D614G, P681R, D950N, G142D
B.1.617.3 40589-V49B5-B S1+S2 ECD T19R, L452R, E484Q, D614G, P681R, D950N, G142D
B.1.617.3 40589-V08B17 S1+S2 ECD T19R, L452R, E484Q, D614G, P681R, D950N, G142D
B.1.617.3 40591-V08H24 S1 T19R, L452R, E484Q, D614G, P681R, G142D
B.1.617.3 40591-V49H3-B S1 T19R, G142D, L452R, E484Q, D614G, P681R

B.1.618

Lineage Catalogue Antigen Mutations
B.1.618 40588-V07E28 N G18S, A119S, A217S, M234I, E367Q
B.1.618 40589-V08B14 S1+S2 ECD Y145del, H146del, E484K, D614G
B.1.618 40591-V08H21 S1 H49Y, Y145del, H146del, E484K, D614G

B.1.620

Lineage Catalogue Antigen Mutations
B.1.62040589-V08B18 (pre-order)S1+S2 ECDP26S, HV69-70del, V126A, Y144del, LLA241-243del, H245Y, S477N, E484K, D614G, P681H, T1027I, D1118H

B.1.2

Lineage Catalogue Antigen Mutations
B.1.2 40588-V07E22 N P67S, P199L

A.23.1

Lineage Catalogue Antigen Mutations
A.23.1 40588-V07E23 N S202N

P.2

Lineage Catalogue Antigen Mutations
P.2 40588-V07E21 N A119S, R203K, G204R, M234I
P.2 40589-V08B19 S1+S2 ECD E484K, F565L, D614G, V1176F
P.2 40591-V08H26 S1 E484K, F565L, D614G, V1176F

P.3

Lineage Catalogue Antigen Mutations
P.3 40592-V08H111 RBD E484K, N501Y

Mink Variant

Lineage Catalogue Antigen Mutations
Mink Variant 40591-V08H8 S1 ΔH69/ΔV70, Y453F, D614G
Mink Variant 40592-V08H80 RBD Y453F
Mink Variant 40592-V31H3 RBD Y453F

More

Catalogue Antigen Mutations Tag
40588-V07E12 N P199L His
40588-V07E13 N A220V His
40588-V07E14 N p67S His
40588-V07E15 N M234L His
40589-V08B4 S1+S2 ECD D614G His
40589-V08B5 S1+S2 ECD HV69-70deletion, D614G, D796H His
40591-V08H20 S1 N343Q His
40591-V02H3 S1 D614G Fc
40591-V08H11 S1 N234Q His
40591-V08H3 S1 D614G His
40591-V08H4 S1 A222V, D614G His
40591-V08H6 S1 L18F, D614G His
40591-V08H9 S1 HV69-70deletion, N439K, D614G His
40591-V08H25(pre-order) S1 P26S, HV69-70del, V126A, Y144del, LLA241-243del, H245Y, S477N, E484K, D614G, P681H His
40592-V08H100 RBD P479L His
40592-V08H101 RBD P479H His
40592-V08H102 RBD N481K His
40592-V08H103 RBD E484A His
40592-V08H104 RBD E484D His
40592-V08H105 RBD N487D His
40592-V08H106 RBD Y449H His
40592-V08H107 RBD G502V His
40592-V08H89 RBD F486A His
40592-V08H92 RBD N440D His
40592-V08H93 RBD F456Y His
40592-V08H94 RBD E471A His
40592-V08H95 RBD E471D His
40592-V08H96 RBD Y473F His
40592-V08H97 RBD A475T His
40592-V08H98 RBD G476A His
40592-V08H99 RBD S477G His
40592-V05H1 RBD V367F mFc
40592-V08H1 RBD V367F His
40592-V08H10 RBD R408I His
40592-V08H11 RBD V341I His
40592-V08H12 RBD Y508H His
40592-V08H14 RBD N439K His
40592-V08H15 RBD V503F His
40592-V08H16 RBD A522V His
40592-V08H19 RBD A372S His
40592-V08H2 RBD N354D His
40592-V08H20 RBD A520S His
40592-V08H21 RBD A522S His
40592-V08H22 RBD D405V, Q414A His
40592-V08H23 RBD Q414E His
40592-V08H24 RBD P384L His
40592-V08H25 RBD A348S His
40592-V08H26 RBD F338L His
40592-V08H27 RBD F377L His
40592-V08H29 RBD P521S His
40592-V08H2-B RBD N354D His
40592-V08H30 RBD T478I His
40592-V08H31 RBD V483I His
40592-V08H32 RBD S359N His
40592-V08H33 RBD K378R His
40592-V08H34 RBD Q409E His
40592-V08H35 RBD I472V His
40592-V08H36 RBD A372T His
40592-V08H37 RBD A344S His
40592-V08H39 RBD A520V His
40592-V08H4 RBD A435S His
40592-V08H40 RBD E406Q His
40592-V08H41 RBD F490S His
40592-V08H42 RBD K378N His
40592-V08H43 RBD N370S His
40592-V08H44 RBD Q414R His
40592-V08H45 RBD S477I His
40592-V08H46 RBD S477N His
40592-V08H47 RBD T385A His
40592-V08H48 RBD T393P His
40592-V08H49 RBD V395I His
40592-V08H5 RBD V483A His
40592-V08H50 RBD A475V His
40592-V08H51 RBD G446V His
40592-V08H52 RBD G485S His
40592-V08H53 RBD G482S His
40592-V08H54 RBD K444R His
40592-V08H55 RBD N440K His
40592-V08H56 RBD E471Q His
40592-V08H57 RBD P479S His
40592-V08H58 RBD A352S His
40592-V08H6 RBD F342L His
40592-V08H62 RBD P337S His
40592-V08H63 RBD P521R His
40592-V08H64 RBD S477R His
40592-V08H68 RBD L455F His
40592-V08H69 RBD K458Q His
40592-V08H7 RBD K458R His
40592-V08H70 RBD N481D His
40592-V08H71 RBD F456L His
40592-V08H72 RBD Y505C His
40592-V08H73 RBD F456E His
40592-V08H74 RBD F486S His
40592-V08H75 RBD N487R His
40592-V08H76 RBD G446S His
40592-V08H78 RBD P499R His
40592-V08H79 RBD V445F His
40592-V08H8 RBD G476S His
40592-V08H83 RBD F490L His
40592-V08H9 RBD W436R His