Pan Influenza A and B Antibodies

Broad Spectrum Antibodies for HA and NP Validated by ELISA and WB Assay

Influenza A
Anti-HA: 86001-RM01
Anti-NP: 40208-R010
Influenza B
Anti-HA: 11053-R004
Anti-NP: 40438-R036

Influenza viruses (both Influenza A and Influenza B) are notorious for their seasonalities and high infectious potentials. Throughout modern history, Influenza A viruses have cast several deadly pandemics, including the historic 1918 Spanish flu (H1N1), Asian flu (H2N2), Hong Kong flu (H3N2), and the more recent H1N1 outbreak in 2009, rendering life losses in the millions.

Due to the high frequency antigenic drift or shift of various influenza viruses, antibody evasion is often observed. Broad-spectrum flu antibodies are appealing due to their ability to bind multiple viral subtypes and potentially compensate for the high mutation rates of influenza viruses.

Sino Biological has developed several broad-spectrum antibodies against Influenza A and B, named Pan Influenza Antibody, to help the study of Influenza biotherapeutic.

Pan Influenza A and B Antibody aganist Hemagglutinin or Nucleoprotein

Pan Influenza A and B Nucleoprotein Antibody

16 Pan Influenza A or B Nucleoprotein antibodies have been developed and validated by ELISA and WB assay.

  Ab Cat# Antigen Type Ig Type Application
Pan Influenza A Nucleoprotein Antibody  40208-MM03 Influenza A H3N2 NP Mouse MAb  Mouse IgG1 WB, ELISA
40208-MM04 Influenza A H3N2 NP Mouse MAb Mouse IgG1 WB, ELISA
40208-R010 Influenza A H3N2 NP Rabbit MAb Rabbit IgG WB, ELISA
40208-R061 Influenza A H3N2 NP Rabbit MAb Rabbit IgG WB, ELISA
40208-R113 Influenza A H3N2 NP Rabbit MAb Rabbit IgG WB, ELISA
40208-R014 Influenza A H3N2 NP Rabbit MAb Rabbit IgG ELISA(Det)
40208-R117 Influenza A H3N2 NP Rabbit MAb Rabbit IgG ELISA(Cap)
40205-MM16 Influenza A H1N1 NP Mouse MAb Mouse IgG2a WB, ELISA(Cap)
40205-MM18 Influenza A H1N1 NP Mouse MAb Mouse IgG2b WB, ELISA(Det)
40205-R063 Influenza A H1N1 NP Rabbit MAb  Rabbit IgG  ELISA, ELISA(Cap)
Pan Influenza B Nucleoprotein Antibody  40438-MM05 Influenza B NP Mouse MAb Mouse IgG1 WB, ELISA (Cap)
40438-MM09 Influenza B NP Mouse MAb Mouse IgG1 WB, ELISA
40438-R004 Influenza B NP Rabbit MAb Rabbit IgG WB, ELISA (Cap)
40438-R016 Influenza B NP Rabbit MAb Rabbit IgG WB, ELISA
40438-R036 Influenza B NP Rabbit MAb Rabbit IgG WB, ELISA (Cap), ELISA (Det)
40438-MM10 Influenza B NP Mouse MAb Mouse IgG1 WB, ELISA(Det)

Lane 1: 40204-V08B | H1N1 (A/Brevig Mission/1/1918)
Lane 2: 40776-V08B | H1N1 (A/Brisbane/02/2018)
Lane 3: 40205-V08B | H1N1 (A/California/07/2009)
Lane 4: 40723-V08B | H1N1 (A/Guangdong-Maonan/SWL1536/2019)
Lane 5: 40724-V08B | H1N1 (A/Hawaii/70/2019)
Lane 6: 40777-V08B | H1N1 (A/Michigan/45/2015)
Lane 7: 11675-V08B | H1N1 (A/Puerto Rico/8/34/Mount Sinai)
Lane 8: 40774-V08B | H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019)
Lane 9: 40033-V08B | H2N2 (A/Ann Arbor/6/1960)
Lane 10: 40207-V08B | H3N2 (A/Aichi/2/1968)
Lane 11: 40778-V08B | H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like)
Lane 12: 40208-V08B | H3N2 (A/Hong Kong/1/1968)
Lane 13: 40753-V08B | H3N2 (A/Hong Kong/2671/2019)
Lane 14: 40754-V08B | H3N2 (A/Hong Kong/45/2019)
Lane 15: 40781-V08B | H3N2 (A/Hong Kong/4801/2014)
Lane 16: 40779-V08B | H3N2 (A/Kansas/14/2017)
Lane 17: 40499-V08B | H3N2 (A/Switzerland/9715293/2013)
Lane 18: 40110-V08B | H7N9 (A/Anhui/1-BALF_RG6/2013)
Lane 19: 40111-V08B | H7N9 (A/Shanghai/2/2013)
Lane 20: 40783-V08B | Influenza B (B/Brisbane/60/2008)
Lane 21: 40782-V08B | Influenza B (B/Colorado/06/2017)
Lane 22: 40438-V08B | Influenza B (B/Florida/4/2006)
Lane 23: 40500-V08B | Influenza B (B/Phuket/3073/2013)
Lane 24: 40755-V08B | Influenza B (B/Washington/02/2019)

WB assays show that two antibodies Cat#:40208-R010 and Cat#:40438-R036 can specifically bind to NP of Influenza A and Influenza B, respectively.

Immunodetection for Pan Influenza Nucleoprotein (NP) Antigens

Influenza viral nucleoprotein (NP) is a structural protein that plays a critical role in virus replication and host adaptation. Antibodies targeting NP proteins are commonly used for immunodetection of influenza viruses in various assays, including ELISA, lateral flow assay (LFA), and direct fluorescent antibody tests. These assays can be used in both research and clinical diagnostics.

Due to the high frequency of antigenic drift or shift among different influenza strains, broad-spectrum influenza antibodies are particularly desired for flu diagnostics. Sino Biological has identified six Pan antibody pairs against the NP protein of Influenza A and Influenza B, respectively. These matched pairs are validated in the sandwich ELISA assay with high sensitivity (pg level). They also demonstrate broad reactivity against a large cohort of flu strains.

Pan Influenza A and B Hemagglutinin Antibody

Because of the distinct role of HA, it is widely used for research on antibodies against influenza. WB and ELISA assay have been performed to confirm the recognition of HA antigens. Results show that rabbit Mab 86001-RM01 and 11053-R004 are broad spectrum antibodies for Influenza A and B, respectively.

Number Antigens ELISA Assay WB Assay ELISA Assay WB Assay Antigen Subtype Antigen Strain Vaccine Strain
86001-RM01  11053-R004 
1 40719-V08H  +++  +++  H1N1  A/Brisbane/02/2018  2019-2020 
2 11055-V08H  +++  +++  A/California/04/2009 
3 11085-V08B  +++  +++  A/California/07/2009  2016-2017, 2015-2016 
4 40717-V08H  +++  +++  A/Guangdong-Maonan/SWL1536/2019  2020-2021 
5 40567-V08H1  +++  +++  A/Michigan/45/2015  2018-2019, 2017-2018 
6 11684-V08H  +++  +++  A/Puerto Rico/8/1934 
7 40787-V08H  +++  +++  (A/Wisconsin/588/2019) / (A/Victoria/2570/2019)  2021-2022 
8 11056-V08H  +++  +++  H3N2  A/Brisbane/10/2007 
9 40789-V08H  +++  +++  A/Cambodia/e0826360/2020  2021-2022 
10 40721-V08H  +++  +++  A/Hong Kong/2671/2019  2020-2021 
11 40765-V08H  +++  +++  A/Hong Kong/45/2019  2020-2021 
12 40555-V08B  +++  +++  A/Hong Kong/4801/2014  2017-2018, 2016-2017 
13 40720-V08H  +++  +++  A/Kansas/14/2017  2019-2020 
14 40580-V08H  +++  +++  A/Singapore/INFIMH-16-0019/2016  2018-2019 
15 40497-V08B  +++  +++  A/Switzerland/9715293/2013  2015-2016 
16 40016-V08B  +++  +++  Influenza B  B/Brisbane/60/2008  2017-2018, 2016-2017, 2015-2016 
17 40581-V08H  +++  +++  B/Colorado/06/2017  2019-2020, 2018-2019 
18 11053-V08H  +++  +++  B/Florida/4/2006 
19 40498-V08B  +++  +++  B/PHUKET/3073/2013  2021-2022, 2020-2021, 2019-2020, 2018-2019, 2017-2018, 2016-2017, 2015-2016 
20 40722-V08H  +++  +++ 
B/Washington/02/2019  2021-2022, 2020-2021 

-: No Effect; +: Medium/Minimal Effect; +++: Strong Effect

Western Blot Analysis of Hemagglutinin Antibodies to aforementioned 20 Influenza Antigens

Antigens for each lane, please refer to the above form.


  • Anti-Influenza A HA rabbit mAb (Cat#: 86001-RM01) at 1:5000 dilution.

  • Anti-Influenza B HA rabbit mAb (Cat#: 11053-R004) at 1:10000 dilution.
 

Featured Influenza Antibodies

Neutralizing Antibodies

H1N1 Anti-HA, Mouse MAb
11055-MM11
Mouse IgG1
 
H5N1 Anti-HA, Mouse MAb
11048-MM01
Mouse IgG1
Anti-HA
68031-H011
Human IgG1
 
H7N7 Anti-HA, Rabbit MAb
11082-R019
Rabbit IgG
 
H10N8 Anti-HA, Mouse MAb
40359-M001
Mouse IgG1
Anti-HA, chimeric MAb
40359-mh001
Human/mouse chimeric IgG

Monoclonal Antibodies

Cat# Target Antibody Type Application Antigen Subtype Antigen Strain
11052-MM06  HA  Mouse MAb  WB, ELISA  H1N1  A/Brisbane/59/2007 
11055-RM10  HA  Rabbit MAb  WB  A/California/04/2009 
11085-MM01  HA  Mouse MAb  ELISA  A/California/07/2009 
11085-MM02  HA  Mouse MAb  WB  A/California/07/2009 
11684-MM03  HA  Mouse MAb  WB, ELISA, ELISA (Det)  A/Puerto Rico/8/1934 
11684-MM05  HA  Mouse MAb  ELISA (Cap)  A/Puerto Rico/8/1934 
11684-R016  HA  Rabbit MAb  ELISA (Cap)  A/Puerto Rico/8/1934 
40010-MM02  M1  Mouse MAb  ELISA  A/Puerto Rico/8/34/Mount Sinai 
40010-R111  M1  Rabbit MAb  ELISA  A/Puerto Rico/8/34/Mount Sinai 
11056-MM01  HA  Mouse MAb  ELISA (Det)  H3N2  A/Brisbane/10/2007 
11056-R006  HA Rabbit MAb  ELISA 
11056-R014  HA Rabbit MAb  ELISA (Cap) 
11056-R104  HA Rabbit MAb  ELISA 
11048-MM14  HA  Mouse MAb  ELISA (Cap)  H5N1  A/Anhui/1/2005 
40158-MM08  HA  Mouse MAb  ELISA  A/chicken/VietNam/NCVD-016/2008 
40398-MM08  HA  Mouse MAb  ELISA  H6N2  A/chicken/Guangdong/C273/2011 
40103-MM06  HA  Mouse MAb  ELISA  H7N9  A/Anhui/1/2013 
11053-MM06  HA  Mouse MAb  WB  Influenza B  B/Florida/4/2006 
11053-MM09  HA  Mouse MAb  ELISA, ELISA (Cap)