Human Coronavirus Nucleocapsid Protein

What is Nucleocapsid Protein?

The nucleocapsid protein (N-protein) is the most abundant protein in coronavirus. The N-protein is a highly immunogenic phosphoprotein, and it is normally very conserved. The N protein of coronavirus is often used as a marker in diagnostic assays. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. The abundance and high hydrophilicity of N protein are supposed to contribute to potent immunity after coroanvirus infection.

Nucleocapsid Protein Function

The N protein constitutes the only protein present in the nucleocapsid. It is composed of two separate domains, an N-terminal domain (NTD) and a C-terminal domain (CTD), both capable of binding RNA in vitro, but each domain uses different mechanisms to bind RNA.

N protein is also heavily phosphorylated, and phosphorylation has been suggested to trigger a structural change enhancing the affinity for viral versus non-viral RNA.

N protein binds the viral genome in a beads-on-a-string type conformation. Two specific RNA substrates have been identified for N protein; the TRSs and the genomic packaging signal. The genomic packaging signal has been found to bind specifically to the second, or C-terminal RNA binding domain.

N protein also binds nsp3, a key component of the replicase complex, and the M protein. These protein interactions likely help tether the viral genome to the replicase-transcriptase complex (RTC), and subsequently package the encapsidated genome into viral particles.
(Ref: Anthony R. Fehr and Stanley Perlman.Coronaviruses: An Overview of Their Replication and Pathogenesis.Methods Mol Biol. 2015)

 

Research Reagents of Nucleocapsid Protein

Sino Biological has developed recombinat proteins, antibodies, genes and other reagents for human coronavirus.

Recombinant Proteins

SARS-CoV-2(2019-nCoV)
SARS-CoV
MERS-CoV
High Purity; Expressed in Insect Cells, closer to Natural Structure;
Cat#:40588-V08B
Purity: >95%
CoV: SARS-CoV-2 (2019-nCoV)

Antibodies

SARS-CoV-2(2019-nCoV)
SARS-CoV
MERS-CoV
High Sensitivity
Cat#:40143-R019
Detection Limit: 0.5ng
CoV: SARS-CoV-2 (2019-nCoV)

Sample: 2019-nCoV NP Protein
(Cat# 40588-V08B)
Lane A: 30ng
Lane B: 10ng
Lane C: 5ng
Lane D: 2ng
Lane E: 1ng
Lane F: 0.5ng

N Protein Detection Kit

Cat#:KIT40588
Assay range: 93.75-6000 pg/mL
Specificity:
Verified by high purity SARS-CoV-2 (2019-nCoV) Nucleocapsid / N Protein (Cat# 40588-V08B) recombinant protein.
Cat#   Antigen More
  N protein

CoV Type MERS-CoV
Expressed Host Insect
Tag His
  N protein

CoV Type SARS-CoV
Expressed Host Insect
Tag His
  N protein

CoV Type SARS-CoV-2 (2019-nCoV)
Expressed Host Insect
Tag His
Cat#   Antigen More
  N protein

CoV Type SARS-CoV
Ab Type Rabbit PAb
Application WB, ELISA, IHC-P, FCM, ICC/IF, IP
  N protein

CoV Type SARS-CoV-2(2019-nCoV)
Ab Type Rabbit MAb
Application WB, ELISA, IHC-P, FCM, ICC/IF, IP
  N protein

CoV Type MERS-CoV
Ab Type Rabbit PAb
Application WB, IHC-P, ICC/IF, IP
  N protein

CoV Type MERS-CoV
Ab Type Mouse MAb
Application WB, ELISA, IHC-P, FCM, ICC/IF, IP
  N protein

CoV Type MERS-CoV
Ab Type Rabbit PAb
Application ELISA, IHC-P, FCM, ICC/IF, IP
  N protein

CoV Type MERS-CoV
Ab Type Rabbit PAb
Application WB, ELISA, IHC-P, FCM, ICC/IF, IP
  N protein

CoV Type SARS-CoV
Ab Type Mouse MAb
Application WB, ELISA, IHC-P, FCM, ICC/IF, IP
  N protein

CoV Type SARS-CoV
Ab Type Rabbit MAb
Application WB, ELISA, IHC-P, FCM, ICC/IF, IP
  N protein

CoV Type SARS-CoV
Ab Type Rabbit PAb
Application ELISA, IHC-P, FCM, ICC/IF, IP
  N protein

CoV Type SARS-CoV-2 (2019-nCoV)
Ab Type Rabbit PAb
Application WB, ELISA, IHC-P, FCM, ICC/IF, IP
Cat#: KIT40588
Assay range: 93.75-6000 pg/mL
Specificity: The kit has been verified by high purity SARS-CoV-2 (2019-nCoV) Nucleocapsid / N Protein (Cat# 40588-V08B) recombinant protein. It can recognize recombinant SARS-CoV Nucleocapsid / N Protein (His Tag) (Cat# 40143-V08B), but no react with recombinant MERS-CoV Nucleoprotein / NP protein (His Tag) (Cat# 40068-V08B).