SARS-CoV-2 (2019-nCoV) Antigen Reagents

SARS-CoV-2 (COVID-19 Virus) Research

SARS-CoV-2 (2019-nCoV, Novel Coronavirus 2019) has led to pneumonia (COVID-19) that sickened over 5.7M people worldwide. Same as all other coronaviruses, the genome of SARS-CoV-2 (2019-nCoV) encodes the spike protein, the envelope protein, the membrane protein, and the nucleocapsid protein.

The spike protein (S-protein) is the common target for neutralizing antibodies and vaccines. Spike protein contains two subunits, S1 and S2. S1 contains a receptor binding domain (RBD), which is responsible for recognizing and binding with the cell surface receptor. S2 subunit contains other basic elements needed for the membrane fusion. SARS-CoV-2 (2019-nCoV) (ref.) can infect the human respiratory epithelial cells through interaction with huamn ACE2. Indeed, the recombinant Spike protein can bind with recombinant ACE2 protein.

The Nucleocapsid Protein (N-protein) is the most abundant protein in coronavirus. The N-protein is a highly immunogenic phosphoprotein, and it is normally very conserved. The N protein of coronavirus is often used as a marker in diagnostic assays.

Sino Biological is the first company in the world to produce recombinant proteins of SARS-CoV-2. We launched the spike protein on Jan 22nd, only 12 days after the sequence is published. Till this day, Sino Biological has developed a large panel of reagents for COVID19 research. The available products include hundreds of antigens (the N protein, S protein, the S1 and S2 subunits of the S protein, and the RBD domain of the S proteins, Plpro, MTase, NSP3, NSP8,NSP9), monoclonal antibodies, ELISA kits, and expression vectors for SARS-CoV-2 and its receptor. These reagents have supported hundreds of research groups around the globe for their vaccine and antibody development works. Sino Biological is also offering pseudovirus-based infection assay services, allowing rapid screening of antibodies and compounds for neutralization potential.

SARS-CoV-2 Antigens

 

SARS-CoV-2 Antibodies

 

Sino Biological has developed a panel of mutated spike proteins to facilitate the study of these variants.

Partial list of Spike Mutations

S1+S2 SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Protein, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P
[ Expressed in HEK293 cell, bind with ACE2 ]
   
S1 SARS-CoV-2 (2019-nCoV) Spike S1 His Protein, D614G
[ Expressed in HEK293 cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein, W436R
[ Expressed in HEK293 cell, bind with ACE2]

Biotinylated SARS-CoV-2 Antigen Proteins

N SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Protein, Biotinylated
[ Expressed in Insect cell ]
N SARS-CoV-2 (2019-nCoV) Nucleocapsid-AVI&His Protein, Biotinylated
[ Expressed in Insect cell ]
   
N SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Recombinant Protein, Biotinylated
[ Expressed in E.coli cell ]
S1+S2 SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein, Biotinylated
[ Expressed in Insect cell ]
   
S1 SARS-CoV-2 (2019-nCoV) Spike S1-His Protein, Biotinylated
[ Expressed in HEK293 cell, bind with ACE2 ]
S1 SARS-CoV-2 (2019-nCoV) Spike S1 NTD-Fc & AVI Protein, Biotinylated
[ Expressed in HEK293 cell ]
   
S1 SARS-CoV-2 (2019-nCoV) Spike S1 NTD-AVI & His Protein, Biotinylated
[ Expressed in HEK293 cell ]
S1 SARS-CoV-2 (2019-nCoV) Spike S1-AVI & His Protein, Biotinylated
[ Expressed in HEK293 cell, bind with ACE2 ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein, Biotinylated
[ Expressed in Insect cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein, Biotinylated
[ Expressed in HEK293 cell, bind with ACE2 ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-AVI & His Protein, Biotinylated
[ Expressed in Insect cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-AVI & His Protein, Biotinylated
[ Expressed in HEK293 cell, bind with ACE2 ]
   
S2 SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Recombinant Protein, Biotinylated
[ Expressed in Insect cell ]

Recombinant SARS-CoV-2 Antigens

N SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Protein
[ Expressed in Insect cell ]
N SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Protein
[ Expressed in E.coli cell ]
   
S1+S2 SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Protein
[ Expressed in Insect cell, bind with ACE2 ]
S1 SARS-CoV-2 (2019-nCoV) Spike S1-His Protein
[ Expressed in HEK293 cell, bind with ACE2 ]
   
S1 SARS-CoV-2 (2019-nCoV) Spike S1-mFc Protein
[ Expressed in HEK293 cell, bind with ACE2 ]
S1 SARS-CoV-2 (2019-nCoV) Spike S1-His Protein
[ Expressed in Insect cell, bind with ACE2 ]
   
S1 SARS-CoV-2 (2019-nCoV) Spike S1-Fc Protein
[ Expressed in HEK293 cell, bind with ACE2 ]
S1 SARS-CoV-2 (2019-nCoV) Spike S1 NTD-Fc & AVI Protein
[ Expressed in HEK293 cell ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein
[ Expressed in HEK293 cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein
[ Expressed in Insect cell, bind with ACE2 ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-Fc Protein
[ Expressed in HEK293 cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein
[ Expressed in HEK293 cell, bind with ACE2 ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-rFc Protein
[ Expressed in HEK293 cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-untagged Protein
[ Expressed in HEK293 cell, bind with ACE2 ]
   
S2 SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Protein
[ Expressed in Insect cell ]
S2 SARS-CoV-2 (2019-nCoV) Spike S2-Fc Protein
[ Expressed in HEK293 cell ]
   
S2 SARS-CoV-2 (2019-nCoV) Spike S2-mFc Protein
[ Expressed in Insect cell ]
S2 SARS-CoV-2 (2019-nCoV) Spike S2 ECD(708S-1207E)-His Recombinant Protein
[ Expressed in HEK293 cell ]
   
ME SARS-CoV-2 (2019-nCoV) Methyltransferase-His Protein
[ Expressed in E.coli cell ]
NSP3 SARS-CoV-2 (2019-nCoV) NSP3-His Protein
[ Expressed in E.coli cell ]
   
NSP8 SARS-CoV-2 (2019-nCoV) NSP8-AVI Protein
[ Expressed in E.coli cell ]
NSP9 SARS-CoV-2 (2019-nCoV) NSP9-AVI&His Protein
[ Expressed in E.coli cell ]
   
3CLpro SARS-CoV-2 (2019-nCoV) 3CLpro-His & AVI Protein
[ Expressed in E.coli cell ]
3CLpro SARS-CoV-2 (2019-nCoV) 3CLpro-His & AVI Protein
[ Expressed in Insect Cell ]
   
3CLpro SARS-CoV-2 (2019-nCoV) 3CLpro-His Protein
[ Expressed in Insect cell ]
Plpro SARS-CoV-2 (2019-nCoV) Plpro-His Protein
[ Expressed in E.coli cell, enzymatic activity ]

The S and N detection kits employ a standard sandwich ELISA, allowing rapid quantification of the N and S antigens.
• All antibodies used in the kits are in-house developed monoclonal antibodies, ensuring batch-to-batch consistency.
• These assays may be used to quantify antigens on the surface of SARS-CoV-2 and/or pseudovirus, as well as antigens expressed recombinantly.

SARS-CoV-2 N Detection Kit
(KIT40588)
More about SARS-CoV-2 Antigen Detection Assay

Sino Biological announced this week that the company has launched the world first coronavirus antigen array under its new brand SinommuneTM. This product is co-developed by Nanommune Inc., a UCI-based biotech company specializing in array technology. This antigen array is specifically designed for high throughput sero-surveillance studies of COVID-19 Coronavirus, and other virus that can lead to upper respiratory infections. The SinommuneTM is constructed by printing recombinant antigens on a nitrocellulose-coated membrane. It can analyze serum antibodies against 66 antigens from 23 types of viruses known to cause respiratory tract infections, including SARS-CoV-2 and the other six coronaviruses.

HCoV-OC43 HCoV-229E SARS-CoV
HCoV-NL63 HCoV-HKU1 MERS-CoV
SARS-CoV-2 Influeza virus RSV
Metapneumovirus Parainfluenza Adenovirus
 

Capacity of Viral Reagents Development

• 2009.5 Launched Recombinant Influenza A H5N1 Hemagglutinin Protein

• 2009.12 Launched Influenza A H5N1 HA ELISA Pair Set and H1N1 HA ELISA Pair Set

• 2012.12 Launched MERS-CoV Spike Protein(S1,S2,S-RBD),NP protein within 50 Days after Sequence Published.

• 2013.4 Completed R&D of H7N9 HA Protein & H7N9 NA Protein in 12 Days.

• 2013.10 Launched SARS CoV Spike Protein, NP Protein.

• 2014.2 Completed R&D of EBOV NP, GP, VP40, VP24 Proteins.

• 2016.6 Completed R&D of ZIKV Envelope, Membrane protein in 14 Days.

• 2020.1 Completed R&D of SARS-CoV-2 (2019-nCoV) Viral Proteins (Spike, S1, S2, RBD protein) in 12 Days.

View Related Viral Products

 

Reference

• Xintian Xu.Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission.SCIENCE CHINA Life Sciences.2020
• Bo Diao, Zeqing Feng.Human Kidney is a Target for Novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection.medRxiv preprint

Novel Immunodiagnostics for COVID-19