IL-6 Protein, Rat, Recombinant

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IL-6 Protein, Rat, Recombinant: Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells.
The ED50 for this effect is typically 1-6 ng/ml.
Protein Construction
A DNA sequence encoding the rat IL6 (NP_036721.1)(Phe25-Thr211) was expressed and purified with an initial Met.
Accession#
Expressed Host
E. coli
Species
Rat
Predicted N Terminal
Met
Molecule Mass
The recombinant rat IL6 consists of 188 amino acids and has a predicted molecular mass of 21.9 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 22 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 40% acetonitrile, 0.1% TFA
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-6 Protein, Rat, Recombinant: Images

Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 1-6 ng/ml.

IL-6 Background Information

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.
Full Name
interleukin 6
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
  • DAPT reverses the Th17/Treg imbalance in experimental autoimmune uveitis in vitro via inhibiting Notch signaling pathway
    Author
    Yin, X;Wei, H;Wu, S;Wang, Z;Liu, B;Guo, L;Bi, H;Guo, D;
    Year
    2019
    Journal
    Int. Immunopharmacol.
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