PSGL-1/CD162 Protein, Mouse, Recombinant

1/1
Price:
Size:
Number:

PSGL-1/CD162 Protein, Mouse, Recombinant: Product Information

Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the extracellular domain of mouse SELPLG (Q62170) (Met1-Cys307) was expressed with six amino acids (LEVLFQ) at the C- terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Leu 18
Molecule Mass
The recombinant mouse SELPLG consists of 297 amino acids and has a calculated molecular mass of 31.2 kDa. The recombinant protein migrates as an approximately 54.9 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PSGL-1/CD162 Protein, Mouse, Recombinant: Images

PSGL-1/CD162 Protein, Mouse, Recombinant: Synonyms

CD162 Protein, Mouse; Psgl-1 Protein, Mouse; Psgl1 Protein, Mouse; Selp1 Protein, Mouse; Selpl Protein, Mouse

PSGL-1/CD162 Background Information

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.
Full Name
selectin P ligand
References
  • 1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
  • 2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
  • 3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
  • 4. Vandendries, E.R .et al., 2004, Thromb. Haemost. 92: 459-466..
  • 5. Pouyani, T. et al., 1995, Cell. 83: 333-343.
  • Engineering Delivery of Nonbiologics Using Poly(lactic-co-glycolic acid) Nanoparticles for Repair of Disrupted Brain Endothelium
    Author
    Inyang, E;Kuriakose, A;Chen, B;Nguyen, K;Cho, M;
    Year
    2020
    Journal
    ACS Omega
    Application
    Conjugation
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.