PD-L1 Protein, Mouse, Recombinant (ECD, hFc Tag)


PD-L1 Protein, Mouse, Recombinant (ECD, hFc Tag): Product Information

> 95 % as determined by SDS-PAGE
< 1.0 EU per μg protein as determined by the LAL method.
1.Measured by its binding ability in a functional ELISA.
2.Immobilized human PD1-His(Cat:10377-H08H) at 10μg/mL (100μL/well) can bind mouse CD274-Fc(Cat:50010-M02H), the EC50 of mouse CD274-Fc is 0.1-0.6μg/mL.
Protein Construction
A DNA sequence encoding the mouse Cd274 (NP_068693.1) (Met1-His239) was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host
HEK293 Cells
Predicted N Terminal
Phe 19
Molecule Mass
The recombinant mouse Cd274 consists 459 amino acids and predicts a molecular mass of 51.6 kDa. As a result of glycosylation, it migrates as an approximately 74.3 kDa band in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PD-L1 Protein, Mouse, Recombinant (ECD, hFc Tag): Images

Measured by its binding ability in a functional ELISA. Immobilized human PD1-His (Cat: 10377-H08H) at 10 μg/mL (100 μL/well) can bind mouse CD274-Fc (Cat: 50010-M02H), the EC50 of mouse CD274-Fc is 0.1-0.6μg/mL.

PD-L1 Protein, Mouse, Recombinant (ECD, hFc Tag): Synonyms

A530045L16Rik Protein, Mouse; B7h1 Protein, Mouse; Pdcd1l1 Protein, Mouse; Pdcd1lg1 Protein, Mouse; Pdl1 Protein, Mouse

PD-L1 Background Information

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Full Name
CD274 molecule
Research Areas
  • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
  • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
  • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
  • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
  • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.
  • PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2
    Xu, X;Hou, B;Fulzele, A;Masubuchi, T;Zhao, Y;Wu, Z;Hu, Y;Jiang, Y;Ma, Y;Wang, H;Bennett, EJ;Fu, G;Hui, E;
    J. Cell Biol.
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