IL-1 alpha/IL1A Protein, Mouse, Recombinant

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IL-1 alpha/IL1A Protein, Mouse, Recombinant: Product Information

Purity
> 92 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-10 pg/mL.
2. Measured by its binding ability in a functional ELISA.
3. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL.
4. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.
The ED50 for this effect is typically 3-15 ng/mL.
Protein Construction
A DNA sequence encoding the mature form of mouse IL1A (NP_034684.2) (Ser 115-Ser 270) was expressed, with an initial Met at the C-terminus.
Accession#
Expressed Host
E. coli
Species
Mouse
Predicted N Terminal
Met
Molecule Mass
The recombinant mouse IL1A consists of 157 amino acids and migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions as predicted.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-1 alpha/IL1A Protein, Mouse, Recombinant: Images

Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 3-15 ng/mL.

IL-1 alpha/IL1A Protein, Mouse, Recombinant: Synonyms

Il-1a Protein, Mouse

IL-1 alpha/IL1A Background Information

IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6, and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13, and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.
Full Name
interleukin 1, alpha
Research Areas
Related Pathways
  • IL1 signaling pathway
    IL1 signaling pathway
References
  • Nicklin MJ,et al. (1994) A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes. Genomics. 19(2):382-4.
  • March CJ, et al. (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature. 315(6021):641-7.
  • Bankers-Fulbright JL, et al. (1996) Interleukin-1 signal transduction. Life Sci. 59(2):61-83.
  • Dinarello CA, et al. (1997) Induction of interleukin-1 and interleukin-1 receptor antagonist. Semin Oncol. 24 (3 Suppl 9):S9-81-S9-93.
  • Downstream of Mutant KRAS, the Transcription Regulator YAP Is Essential for Neoplastic Progression to Pancreatic Ductal Adenocarcinoma
    Author
    Zhang, W;Nandakumar, N;Shi, Y;Manzano, M;Smith, A;Graham, G;Gupta, S;Vietsch, EE;Laughlin, SZ;Wadhwa, M;Chetram, M;Joshi, M;Wang, F;Kallakury, B;Toretsky, J;Wellstein, A;Yi, C;
    Year
    2014
    Journal
    Sci Signal
    Application
    cell-based
  • Axl deficiency promotes the neuroinvasion of Japanese encephalitis virus by enhancing IL-1α production from pyroptotic macrophages
    Author
    Wang, ZY;Zhen, ZD;Fan, DY;Qin, CF;Han, DS;Zhou, HN;Wang, PG;An, J;
    Year
    2020
    Journal
    J. Virol.
    Application
    cell cilture
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