CD45 Protein, Mouse, Recombinant (aa 453-1152)

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CD45 Protein, Mouse, Recombinant (aa 453-1152): Product Information

Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. The specific activity was determined to be 9168 nmol/min/mg using p-nitrophenyl phosphate as substrate.
2. Measured by its binding ability in a functional ELISA. Immobilized mouse PTPRC (453-1152) at 10 μg/ml (100 μl/well) can bind biotinylated human Galectin-1 (Cat:10290-HNAE-E) with a linear range of 0.31-2.5 μg/ml.
Protein Construction
A DNA sequence encoding the mouse PTPRC (AAA39458.1) (Arg453-Ser1152) was fused with five amino acids (DDDDK) at the C-terminus was expressed and purified.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
Mouse
Predicted N Terminal
Arg 453
Molecule Mass
The recombinant mouse PTPRC consists of 706 amino acids and has a calculated molecular mass of 81 kDa. The recombinant protein migrates as an approximately 93 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, 3mM DTT, pH 7.4.
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD45 Protein, Mouse, Recombinant (aa 453-1152): Images

CD45 Protein, Mouse, Recombinant (aa 453-1152): Synonyms

B220 Protein, Mouse; Cd45 Protein, Mouse; CD45R Protein, Mouse; L-CA Protein, Mouse; loc Protein, Mouse; Ly-5 Protein, Mouse; Lyt-4 Protein, Mouse; T200 Protein, Mouse

CD45 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.
Full Name
protein tyrosine phosphatase, receptor type, C
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Irie-Sasaki J, et al. (2001) CD45 is a JAK phosphatase and negatively regulates cytokine receptor signaling. Nature. 409: 349-54.
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