CD32B/Fcgr2b Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated

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CD32B/Fcgr2b Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Product Information

Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Biotin/Protein Ratio
0.5-1 as determined by the HABA assay.
Activity
Measured by its ability to bind human IgG1 in a functional ELISA.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FGGR2B (NP_001070657.1) as fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Thr 40
Molecule Mass
The secreted recombinant mouse FGGR2B consists of 212 amino acids and has a predicted molecular mass of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGGR2B is approximately 38-43 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD32B/Fcgr2b Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Images

Measured by its ability to bind human IgG1 in a functional ELISA.

CD32B/Fcgr2b Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Synonyms

AI528646 Protein, Mouse; CD32 Protein, Mouse; F630109E10Rik Protein, Mouse; Fcgr2 Protein, Mouse; Fcgr2a Protein, Mouse; FcgRII Protein, Mouse; Fcr-2 Protein, Mouse; Fcr-3 Protein, Mouse; fcRII Protein, Mouse; Fc[g]RII Protein, Mouse; Ly-17 Protein, Mouse; Ly-m20 Protein, Mouse; LyM-1 Protein, Mouse

CD32B/Fcgr2b Background Information

Full Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • Conserved FcγR- glycan discriminates between fucosylated and afucosylated IgG in humans and mice.
    Author
    Dekkers, G;Bentlage, AEH;Plomp, R;Visser, R;Koeleman, CAM;Beentjes, A;Mok, JY;van Esch, WJE;Wuhrer, M;Rispens, T;Vidarsson, G;
    Year
    2017
    Journal
    Mol. Immunol.
  • Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation
    Author
    Zeeuw van der Laan, EAN;van der Velden, S;Bentlage, AEH;Larsen, MD;van Osch, TLJ;Mok, JY;Brasser, G;Geerdes, DM;Koeleman, CAM;Nouta, J;Semple, JW;Porcelijn, L;van Esch, WJE;Wuhrer, M;van der Schoot, CE;Vidarsson, G;Kapur, R;
    Year
    2020
    Journal
    Blood Adv
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