CD32B/Fcgr2b Protein, Mouse, Recombinant (His Tag)

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CD32B/Fcgr2b Protein, Mouse, Recombinant (His Tag): Product Information

Purity
> 98 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA.
Immobilized mouse FCGR2B-His (CD32) (Cat:50030-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.13-0.29 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FCGR2B (NP_001070657.1) precursor was expressed with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Thr 40
Molecule Mass
The secreted recombinant mouse FCGR2B consists of 189 amino acids and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR2B is approximately 34 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD32B/Fcgr2b Protein, Mouse, Recombinant (His Tag): Images

Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR2B-His (CD32) (Cat: 50030-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.13-0.29 μg/ml.

CD32B/Fcgr2b Protein, Mouse, Recombinant (His Tag): Synonyms

AI528646 Protein, Mouse; CD32 Protein, Mouse; F630109E10Rik Protein, Mouse; Fcgr2 Protein, Mouse; Fcgr2a Protein, Mouse; FcgRII Protein, Mouse; Fcr-2 Protein, Mouse; Fcr-3 Protein, Mouse; fcRII Protein, Mouse; Fc[g]RII Protein, Mouse; Ly-17 Protein, Mouse; Ly-m20 Protein, Mouse; LyM-1 Protein, Mouse

CD32B/Fcgr2b Background Information

Full Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
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    Author
    Nguyen, L;Montrasio, F;Pattamatta, A;Tusi, SK;Bardhi, O;Meyer, KD;Hayes, L;Nakamura, K;Banez-Coronel, M;Coyne, A;Guo, S;Laboissonniere, LA;Gu, Y;Narayanan, S;Smith, B;Nitsch, RM;Kankel, MW;Rushe, M;Rothstein, J;Zu, T;Grimm, J;Ranum, LPW;
    Year
    2019
    Journal
    Neuron
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