MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)

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MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag): Product Information

Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA.
1.Immobilized Sh(2C-EMC)(1-1297)(Cat:40069-V08B) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat:10688-HNCH),The EC50 of can biotinylated DPP4 (Cat:10688-HNCH) is 0.02-0.04 ug/mL.
2.Immobilized Sh(2C-EMC)(1-1297)(Cat:40069-V08B) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat:10688-H01H),The EC50 of can biotinylated Fc-DPP4 (Cat:10688-H01H) is 0.01-0.02 ug/mL.
Protein Construction
A DNA sequence encoding the extracellular domain of spike protein MERS-CoV (AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
MERS-CoV
Predicted N Terminal
Tyr 18
Molecule Mass
The recombinant extracellular domain of spike protein MERS-CoV comprises 1291 amino acids and has a predicted molecular mass of 142.52 kDa.
Formulation
Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag): Images

Measured by its binding ability in a functional ELISA. 1. Immobilized Sh(2C-EMC)(1-1297)(Cat: 40069-V08B) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat: 10688-HNCH), The EC50 of can biotinylated DPP4 (Cat: 10688-HNCH) is 0.02-0.04 μg/mL. 2. Immobilized Sh(2C-EMC)(1-1297)(Cat: 40069-V08B) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H), The EC50 of can biotinylated Fc-DPP4 (Cat: 10688-H01H) is 0.01-0.02 μg/mL.

MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag): Synonyms

coronavirus s1 Protein, MERS-CoV; coronavirus s2 Protein, MERS-CoV; coronavirus spike Protein, MERS-CoV; cov spike Protein, MERS-CoV; ncov RBD Protein, MERS-CoV; ncov s1 Protein, MERS-CoV; ncov s2 Protein, MERS-CoV; ncov spike Protein, MERS-CoV; RBD Protein, MERS-CoV; S Protein, MERS-CoV; s1 Protein, MERS-CoV; Spike RBD Protein, MERS-CoV

Coronavirus spike Background Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
References
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

2019-nCoV Coronavirus spike Proteins

  • Characterization of anti-MERS-CoV antibodies against various recombinant structural antigens of MERS-CoV in an imported case in China
    Author
    Wang, W;Wang, H;Deng, Y;Song, T;Lan, J;Wu, G;Ke, C;Tan, W;
    Year
    2016
    Journal
    Emerg Microbes Infect
    Application
    ELISA
  • A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV
    Author
    Fan, C;Wu, X;Liu, Q;Li, Q;Liu, S;Lu, J;Yang, Y;Cao, Y;Huang, W;Liang, C;Ying, T;Jiang, S;Wang, Y;
    Year
    2018
    Journal
    Viruses
    Application
    biopanning
  • Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of middle east respiratory syndrome coronavirus
    Author
    Deng, Y;Lan, J;Bao, L;Huang, B;Ye, F;Chen, Y;Yao, Y;Wang, W;Qin, C;Tan, W;
    Year
    2018
    Journal
    Emerg Microbes Infect
    Application
    immunization
  • Development and validation of different indirect ELISAs for MERS-CoV serological testing
    Author
    Hashem, AM;Al-Amri, SS;Al-Subhi, TL;Siddiq, LA;Hassan, AM;Alawi, MM;Alhabbab, RY;Hindawi, SI;Mohammed, OB;Amor, NS;Alagaili, AN;Mirza, AA;Azhar, EI;
    Year
    2019
    Journal
    J. Immunol. Methods
    Application
    ELISA
  • Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein
    Author
    Ozharovskaia, TA;Zubkova, OV;Dolzhikova, IV;Gromova, AS;Grousova, DM;Tukhvatulin, AI;Popova, O;Shcheblyakov, DV;Scherbinin, DN;Dzharullaeva, AS;Erokhova, AS;Shmarov, MM;Loginova, SY;Borisevich, SV;Naroditsky, BS;Logunov, DY;Gintsburg, AL;
    Year
    2019
    Journal
    Acta Naturae
    Application
    ELISA
  • Analysis of SARS-CoV-2 Antibodies in COVID-19 Convalescent Blood using a Coronavirus Antigen Microarray
    Author
    De Assis, RR;Jain, A;Nakajima, R;Jasinskas, A;
    Year
    2020
    Journal
    BioRxiv
  • Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray
    Author
    Khan, S;Nakajima, R;Jain, A;De Assis, RR;Jasinskas, A;
    Year
    2020
    Journal
    BioRxiv
  • Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient
    Author
    Zhou, D;Duyvesteyn, H;Chen, C;Huang, C;Chen, T;Shih, S;Lin, Y;Cheng, C;Cheng, S;Huang, Y;Lin, T;Ma, C;Huo, J;Carrique, L;Malinauskas, T;Ruza, R;Shah, P;Tan, T;Rijal, P;Donat, R;Godwin, K;Buttigieg, K;Tree, J;Radecke, J;Paterson, N;Supasa, P;Mongkolsapaya, J;Screaton, G;Carroll, M;Jaramillo, J;Knight, M;James, W;Owens, R;Naismith, J;Townsend, A;Fry, E;Zhao, Y;Ren, J;Stuart, D;Huang, K;
    Year
    2020
    Journal
    bioRxiv
  • Spike protein up-regulates inflammatory axis of both thromboinflammation and leukotriene in severe COVID-19
    Author
    Tang, X;Fang, M;Zhang, J;Liao, Z;Cheng, R;Xu, K;Zhao, H;Wang, J;Tan, Z;Wang, G;Kamau, P;Zhang, Z;Mwangi, J;Lu, Q;Deng, G;Lai, R;
    Year
    2020
    Journal
    Research Square
    Application
    animal immunization
  • Characterization of heparin and severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) spike glycoprotein binding interactions
    Author
    Kim, SY;Jin, W;Sood, A;Montgomery, DW;Grant, OC;Fuster, MM;Fu, L;Dordick, JS;Woods, RJ;Zhang, F;Linhardt, RJ;
    Year
    2020
    Journal
    Antiviral Res.
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