PDGFRB Protein, Human, Recombinant

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PDGFRB Protein, Human, Recombinant: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA. Immobilized human PDGFRβ at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB (Cat:90030-C04H), The EC50 of Cynomolgus PDGFB (Cat:90030-C04H) is 3-7 ng/ml.
Protein Construction
A DNA sequence encoding the extracellular domain of human PDGFRβ (NP_002600.1) (Met 1-Lys 531) was expressed with six amino acids (ENLYFQ) at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Leu 33
Molecule Mass
The recombinant human PDGFRβ consists of 506 amino acids and predicts a molecular mass of 57.1 KDa. It migrates as an approximately 92-116 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PDGFRB Protein, Human, Recombinant: Images

Measured by its binding ability in a functional ELISA. Immobilized human PDGFRβ at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB (Cat: 90030-C04H), The EC50 of Cynomolgus PDGFB (Cat: 90030-C04H) is 3-7 ng/ml.

PDGFRB Protein, Human, Recombinant: Synonyms

CD140B Protein, Human; IBGC4 Protein, Human; IMF1 Protein, Human; JTK12 Protein, Human; KOGS Protein, Human; PDGFR Protein, Human; PDGFR-1 Protein, Human; PDGFR1 Protein, Human; PENTT Protein, Human

PDGFRB Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.
Full Name
platelet-derived growth factor receptor, beta polypeptide
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Suzuki S, et al. (2010) Clinicopathological significance of platelet-derived growth factor (PDGF)-B and vascular endothelial growth factor-A expression, PDGF receptor- phosphorylation, and microvessel density in gastric cancer. BMC cancer. 10: 659.
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