Nectin-2 Protein, Human, Recombinant (hFc Tag): Product Information
> 95 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
1. Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 μg/ml (100 μl/well) can bind human CD112 with a linear range of 6.4-800 ng/ml. 2. Immobilized PVRIG Protein, Cynomolgus, Rhesus, Recombinant (His Tag)(Cat:90971-C08H) at 2 μg/ml (100 μl/well) can bind Nectin-2 Protein, Human, Recombinant (hFc Tag)(Cat:10005-H02H), the EC50 of Nectin-2 Protein, Human, Recombinant (hFc Tag)is 0.2-1.2 ng/mL.
A DNA sequence encoding the extracellular domain (Met 1-Leu 360) of human CD112 (NP_002847.1) was fused with the Fc region of human IgG1 at the C-terminus.
The recombinant mature human CD112/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 567 amino acids and has a calculated molecular mass of 62.3 kDa. As a result of glycosylation, the rh CD112/Fc monomer migrates as an approximately 70-80 kDa protein in SDS-PAGE under reducing conditions.
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
poliovirus receptor-related 2 (herpesvirus entry mediator B)
Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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