Interferon Gamma Protein, Human, Recombinant

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Interferon Gamma Protein, Human, Recombinant: Product Information

Purity
> 92 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV) . The EC50 for this effect is typically 0.1-0.5 ng/mL.
Protein Construction
A DNA sequence encoding the human γ-IFN (NP_000610.2) (Met 1-Gln 166) was expressed and purified.
Accession#
Expressed Host
CHO Stable Cells
Species
Human
Predicted N Terminal
Gln 24
Molecule Mass
The secreted recombinant human γ-IFN consists of 143 amino acids and has a predicted molecular mass of 16.7 kDa. γ-IFN migrates as two bands with apparent molecular mass of 21&25 kDa probably due to different glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Interferon Gamma Protein, Human, Recombinant: Images

Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV) . The EC50 for this effect is typically 0.1-0.5 ng/mL.

Citation List

Interferon Gamma Protein, Human, Recombinant: Synonyms

IFG Protein, Human; IFI Protein, Human; IFN gamma Protein, Human; Interferon Gamma Protein, Human

Interferon Gamma Background Information

IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
Full Name
interferon, gamma
Research Areas
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
References
  • Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63.
  • Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
  • Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
  • Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
  • Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
  • Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.
  • Nuclear actin regulates inducible transcription by enhancing RNA polymerase II clustering
    Author
    Wei, M;Fan, X;Ding, M;Li, R;Shao, S;Hou, Y;Meng, S;
    Year
    2019
    Journal
    bioRxiv
    Application
    cell-based
  • CD137 ligand feedback upregulates PD-L1 expression on lung cancer via T cell production of IFN-γ
    Author
    Wang, H;Yan, Z;Hao, J;Yang, B;Wang, J;Yi, L;Wang, X;Li, S;Zhang, H;Zhang, S;
    Year
    2019
    Journal
    Thorac Cancer
    Application
    cell-based
  • A Tumor-Specific Super-Enhancer Drives Immune Evasion by Guiding Synchronous Expression of PD-L1 and PD-L2
    Author
    Xu, Y;Wu, Y;Zhang, S;Ma, P;Jin, X;Wang, Z;Yao, M;Zhang, E;Tao, B;Qin, Y;Chen, H;Liu, A;Chen, M;Xiao, M;Lu, C;Mao, R;Fan, Y;
    Year
    2019
    Journal
    Cell Rep
    Application
    cell-based
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