IL1R1 Protein, Human, Recombinant (hFc Tag): Product Information
> 90 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
1. Measured by its binding ability in a functional ELISA. Immobilized human IL1-beta at 20 μg/ml (100 μl/well) can bind human IL1R1 Fc chimera with a linear range of 6.4-160 ng/ml. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized human IL1B-his at 10 μg/mL (100 μl/well) can bind human IL1R1-Fc. The EC50 of human IL1R1-Fc is 0.198 μg/mL. 4. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1-Fc. The EC50 of human IL1R1-Fc is 0.48 μg/mL. 5. Measured by its ability to bind canine IL1B in a functional ELISA. 6. Measured by its ability to bind mouse IL1B in a functional ELISA.
A DNA sequence encoding the extracellular domain of human IL1R1 (NP_000868.1) (Met 1-Thr 332) was fused with human IgG1 Fc region at the C-terminus.
The recombinant human IL1R1/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 553 amino acids after removal of the signal peptide and has a predicted molecular mass of 63 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 80-90 kDa due to glycosylation.
Lyophilized from sterile PBS, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.
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