IL-18Rβ / IL-18RAcP Protein, Human, Recombinant (His Tag)

1/1
Price:
Size:
Number:

IL-18Rβ / IL-18RAcP Protein, Human, Recombinant (His Tag): Product Information

Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to bind biotinylated Cynomolgus IL18 (Cat:90011-C07E) in a functional ELISA.
Protein Construction
A DNA sequence encoding the human IL18RAP (NP_003844.1) (Met 1-Gly 357) was expressed, fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Phe 20
Molecule Mass
The recombinant human IL18RAP consists of 349 amino acids and has a calculated molecular mass of 40 kDa. As a result of glycosylation, the apparent molecular mass of rh IL18RAP is approximately 50-55 kDa in reduced SDS-PAGE.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-18Rβ / IL-18RAcP Protein, Human, Recombinant (His Tag): Images

IL-18Rβ / IL-18RAcP Protein, Human, Recombinant (His Tag): Synonyms

ACPL Protein, Human; CD218b Protein, Human; CDw218b Protein, Human; IL-18R-beta Protein, Human; IL-18RAcP Protein, Human; IL-18Rbeta Protein, Human; IL-1R-7 Protein, Human; IL-1R7 Protein, Human; IL-1RAcPL Protein, Human; IL18RB Protein, Human

IL-18Rβ / IL-18RAcP Background Information

Interleukin 18 receptor accessory protein, also known as IL18RAP and CDw218b (cluster of differentiation w218b), is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand-binding subunit of the IL18 receptor. The coexpression of IL18R1 and this protein is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18. IL18RAP is required for the high-affinity binding of interleukin 18 (IL-18) to its receptor complex. IL18RAP together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Two putative isoforms of IL18RAP were detected and the ratios and total levels of these isoforms may contribute to the etiology of coeliac disease. IL18R1 and IL18RAP polymorphisms have been found associated with diseases such as schizophrenia, HSV1 seropositivity, and atopic asthma. Analysis of IL18R1 and IL18RAP SNPs in 5 European prospective cohorts suggests that the variability of these genes are unlikely to contribute to modulate the risk of CVD in European populations.
Full Name
interleukin 18 receptor accessory protein
Research Areas
Related Pathways
  • IL1 signaling pathway
    IL1 signaling pathway
References
  • Zhernakova A, et al.. (2008) Genetic analysis of innate immunity in Crohn's disease and ulcerative colitis identifies two susceptibility loci harboring CARD9 and IL18RAP. Am J Hum Genet. 82(5): 1202-10.
  • Grisoni ML, et al.. (2009) Lack of association between polymorphisms of the IL18R1 and IL18RAP genes and cardiovascular risk: the MORGAM Project. BMC Med Genet. 10: 44.
  • Koskinen LL, et al.. (2009) Association study of the IL18RAP locus in three European populations with coeliac disease. Hum Mol Genet. 18(6): 1148-55.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.