IL-6 Protein, Human, Recombinant

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IL-6 Protein, Human, Recombinant: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Immobilized Human IL-6 (Cat:10395-HNAE) at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc(Cat:10398-H02H), the EC50 of Human IL-6R hFc(Cat:10398-H02H) is 2.0-16.0 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.6 ng/mL.
Protein Construction
A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human IL6 consists of 184 amino acids and migrates with an apparent molecular mass of 20.95 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-6 Protein, Human, Recombinant: Images

Immobilized Human IL-6 (Cat:10395-HNAE) at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc(Cat:10398-H02H), the EC50 of Human IL-6R hFc(Cat:10398-H02H) is 2.0-16.0ng/mL.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.6 ng/mL.

IL-6 Protein, Human, Recombinant: Synonyms

BSF2 Protein, Human; HGF Protein, Human; HSF Protein, Human; IFNB2 Protein, Human; IL-6 Protein, Human; Interleukin-6 Protein, Human

IL-6 Background Information

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.
Full Name
interleukin 6
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
  • Identification of a natural product-like STAT3 dimerization inhibitor by structure-based virtual screening
    Author
    LJ Liu, KH Leung, DSH Chan, YT Wang, DL Ma…
    Year
    2014
    Journal
    Cell Death and Disease
    Application
    induce the phosphorylation of STAT3
  • The anti-inflammatory NHE-06 restores antitumor immunity by targeting NF-κB/IL-6/STAT3 signaling in hepatocellular carcinoma
    Author
    Lu, X;Wo, G;Li, B;Xu, C;Wu, J;Jiang, C;Wei, J;
    Year
    2018
    Journal
    Biomed. Pharmacother.
    Application
    cell-based
  • Interleukin-6 Therapy Improves Intestinal Recovery Following Ischemia
    Author
    Te Winkel, JP;Drucker, NA;Morocho, BS;Shelley, WC;Markel, TA;
    Year
    2019
    Journal
    J. Surg. Res.
    Application
    animal-based
  • Ectosomal PKM2 Promotes HCC by Inducing Macrophage Differentiation and Remodeling the Tumor Microenvironment
    Author
    Hou, PP;Luo, LJ;Chen, HZ;Chen, QT;Bian, XL;Wu, SF;Zhou, JX;Zhao, WX;Liu, JM;Wang, XM;Zhang, ZY;Yao, LM;Chen, Q;Zhou, D;Wu, Q;
    Year
    2020
    Journal
    Mol. Cell
    Application
    cell culture
  • Iron promotes breast cancer cell migration via IL-6/JAK2/STAT3 signaling pathways in a paracrine or autocrine IL-6-rich inflammatory environment
    Author
    Cheng, M;Liu, P;Xu, L;
    Year
    2020
    Journal
    Journal of Inorganic Biochemistry
    Application
    cell culture
  • Engineering mesenchymal stem cells with neutralizing and anti- inflammation dual-capability against SARS-CoV-2 infection
    Author
    Zhang, X;Han, P;Wang, H;Xu, Y;Li, F;Li, M;Fan, L;Zhang, H;Dai, Q;Lin, H;Qi, X;Liang, J;Wang, X;Yang, X;
    Year
    2020
    Journal
    Research Square
    Application
    TF–1 proliferation assay
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