IL-1 beta Protein, Human, Recombinant

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IL-1 beta Protein, Human, Recombinant: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL.
2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.
3. Using the Octet RED System, the affinity constant (Kd) of IL1R2 Protein, Human, Recombinant (Fc Tag) (Cat. 10111-H02H) bound IL-1 beta Protein, Human, Recombinant (Cat. 10139-HNAE) was 6.0 nM.
Protein Construction
A DNA sequence encoding the mature form of human IL1β (NP_000567.1) (Ala 117-Ser 269) was expressed.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human IL1β consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-1 beta Protein, Human, Recombinant: Images

Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta (Cat:10139-HNAE) at 2 μg/ml (100 μl/well) can bind Human IL1R2 hFc(Cat:10111-H02H), the EC50 of Human IL1R2 hFc is 150-600 ng/mL.
Using the Octet RED System, the affinity constant (Kd) of IL1R2 Protein, Human, Recombinant (Fc Tag) (Cat. 10111-H02H) bound IL-1 beta Protein, Human, Recombinant (Cat. 10139-HNAE) was 6.0 nM.
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.

IL-1 beta Protein, Human, Recombinant: Synonyms

IL-1 Protein, Human; IL-1 beta Protein, Human; IL-1B Protein, Human; IL1-BETA Protein, Human; IL1F2 Protein, Human

IL-1 beta Background Information

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of a successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Full Name
interleukin 1, beta
Research Areas
Related Pathways
  • IL1 signaling pathway
    IL1 signaling pathway
References
  • Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16.
  • Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42.
  • Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004.
  • Inflammatory human umbilical cord-derived mesenchymal stem cells promote stem cell-Like Characteristics of Cancer Cells in an IL-1β-Dependent Manner
    Author
    Luo, X;Huang, S;He, N;Liu, C;Chen, Y;Liu, Y;Mi, X;Li, N;
    Year
    2018
    Journal
    BioMed Research International
    Application
    cell-based
  • IL-1β from M2 macrophages promotes migration and invasion of ESCC cells enhancing epithelial-mesenchymal transition and activating NF-κB signaling pathway
    Author
    Zhou, J;Zheng, S;Liu, T;Liu, Q;Chen, Y;Tan, D;Ma, R;Lu, X;
    Year
    2018
    Journal
    J. Cell. Biochem.
    Application
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    Author
    Hou, PP;Luo, LJ;Chen, HZ;Chen, QT;Bian, XL;Wu, SF;Zhou, JX;Zhao, WX;Liu, JM;Wang, XM;Zhang, ZY;Yao, LM;Chen, Q;Zhou, D;Wu, Q;
    Year
    2020
    Journal
    Mol. Cell
  • Development and application of an antibody that binds to interleukin-1β of various mammalian species for the treatment of inflammatory diseases
    Author
    Oh, YS;Kwak, MK;Kim, K;Cho, EH;Jang, SE;
    Year
    2020
    Journal
    Biochem. Biophys. Res. Commun.
    Application
    biopanning;Elisa;
  • Endometrial inflammasome activation accompanies menstruation and may have implications for systemic inflammatory events of the menstrual cycle
    Author
    Azlan, A;Salamonsen, LA;Hutchison, J;Evans, J;
    Year
    2020
    Journal
    Hum. Reprod.
    Application
    Elisa
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