FGF2 Protein, Human, Recombinant
Cat: 10014-HNAE
FGF2 Protein, Human, Recombinant: Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method
Activity
Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts.
The ED50 for this effect is typically 0.02-0.1 ng/ml.
The ED50 for this effect is typically 0.02-0.1 ng/ml.
Protein Construction
A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro 143-Ser 288) was expressed, with an additional Met at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human bFGF consists of 147 amino acids and predicts a molecular mass of 16.5 kDa.
Formulation
Lyophilized from sterile 20mM Tris, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
FGF2 Protein, Human, Recombinant: Images
Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.1 ng/ml.
FGF2 Protein, Human, Recombinant: Synonyms
BFGF Protein, Human; FGF-2 Protein, Human; FGFB Protein, Human; HBGF-2 Protein, Human
FGF2 Background Information
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.
bFGF/FGF2 Protein & Antibody Products
Full Name
fibroblast growth factor 2 (basic)
Research Areas
References
- Takayama S,et al. (2001) Periodontal regeneration by FGF-2 (bFGF) in primate models. J Dent Res. 80(12): 2075-9.
- Niu YJ, et al. (2004) Therapeutic effect of bFGF on retina ischemia-reperfusion injury. Chin Med J (Engl). 117(2): 252-7.
- Zhang Y,et al. (2004) Expression of aFGF, bFGF, and FGFR1 in ovarian epithelial neoplasm. Chin Med J (Engl). 117(4): 601-3.
Mouse FGF2 Proteins
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Establishment of a Rabbit Oct4 Promoter-Based EGFP Reporter SystemAuthorQuan, L;Chen, Y;Song, J;Yan, Q;Zhang, Q;Lai, S;Fan, N;Xin, J;Zou, Q;Lai, L;Year2014JournalPLoS ONEApplicationculture
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A lab-centred approach for initiating early stage regeneration of dental pulp using specialised alginate biomimetic microenvironmentsAuthorBhoj, M;Year2015JournalHKU Theses Online (HKUTO)
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Expression of functional recombinant human fibroblast growth factor 8b and its protective effects on MPP(+)-lesioned PC12 cellsAuthorChen, N;Ma, J;Zhao, Y;Wu, M;Yang, H;Gong, W;Chao, J;Li, X;Year2015JournalAppl. Microbiol. Biotechnol.Applicationcell-based
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Receptor FGFRL1 does not promote cell proliferation but induces cell adhesionAuthorYang, X;Steinberg, F;Zhuang, L;Bessey, R;Trueb, B;Year2016JournalInt. J. Mol. Med.Applicationcell-based
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PRC2 specifies ectoderm lineages and maintains pluripotency in primed but not naïve ESCsAuthorShan, Y;Liang, Z;Xing, Q;Zhang, T;Wang, B;Tian, S;Huang, W;Zhang, Y;Yao, J;Zhu, Y;Huang, K;Liu, Y;Wang, X;Chen, Q;Zhang, J;Shang, B;Li, S;Shi, X;Liao, B;Zhang, C;Lai, K;Zhong, X;Shu, X;Wang, J;Yao, H;Chen, J;Pei, D;Pan, G;Year2017JournalNat Commun
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Androgen Promotes Differentiation of PLZF+ Spermatogonia pool via Indirect Regulatory PatternAuthorWang, J;Li, J;Gu, Y;Xia, Q;Song, W;Zhang, X;Yang, Y;Wang, W;Li, H;Zou, K;Year2018JournalbioRxivApplicationcell-based
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In Vitro Modeling of Human Germ Cell Development Using Pluripotent Stem CellsAuthorZhao, Y;Ye, S;Liang, D;Wang, P;Fu, J;Ma, Q;Kong, R;Shi, L;Gong, X;Chen, W;Ding, W;Yang, W;Zhu, Z;Chen, H;Sun, X;Zhu, J;Li, Z;Wang, Y;Year2018JournalStem Cell ReportsApplicationcell-based (hESC/iPS)
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Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and MyelinationAuthorLiu, Z;Yan, M;Liang, Y;Liu, M;Zhang, K;Shao, D;Jiang, R;Li, L;Wang, C;Nussenzveig, DR;Zhang, K;Chen, S;Zhong, C;Mo, W;Fontoura, BMA;Zhang, L;Year2019JournalNeuronApplicationcell-based
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A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body MetabolismAuthorOu, Z;Ma, Y;Sun, Y;Zheng, G;Wang, S;Xing, R;Chen, X;Han, Y;Wang, J;Lu, QR;Zhao, TJ;Chen, Y;Year2019JournalCell RepApplicationcell-based
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Characterization of porcine extraembryonic endoderm cellsAuthorShen, QY;Yu, S;Zhang, Y;Zhou, Z;Zhu, ZS;Pan, Q;Lv, S;Niu, HM;Li, N;Peng, S;Liao, MZ;Wang, HY;Lei, AM;Miao, YL;Liu, ZH;Hua, JL;Year2019JournalCell Prolif.
