EGFR Protein, Human, Recombinant (ECD, hFc Tag)


EGFR Protein, Human, Recombinant (ECD, hFc Tag): Product Information

> 97 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human EGF at 10 μg/ml (100 μl/well) can bind human EGFR with a linear range of 0.64-400 ng/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host
HEK293 Cells
Predicted N Terminal
Leu 25
Molecule Mass
The recombinant human EGFR/Fc chimera is a homodimeric protein. The reduced monomer consists of 860 amino acids and has a calculated molecular mass of 95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110-140 kDa protein in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EGFR Protein, Human, Recombinant (ECD, hFc Tag): Images

Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 10 μg/ml (100 μl/well) can bind human EGFR with a linear range of 0.64-400 ng/ml.

EGFR Protein, Human, Recombinant (ECD, hFc Tag): Synonyms

ERBB Protein, Human; ERBB1 Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
Related Pathways
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains
    Alvarez-Cienfuegos, A;Nuñez-Prado, N;Compte, M;Cuesta, AM;Blanco-Toribio, A;Harwood, SL;Villate, M;Merino, N;Bonet, J;Navarro, R;Muñoz-Briones, C;Sørensen, KM;Mølgaard, K;Oliva, B;Sanz, L;Blanco, FJ;Alvarez-Vallina, L;
    Sci Rep
  • Plasma-Generated OH Radical Production for Analyzing Three-Dimensional Structure in Protein Therapeutics
    Minkoff, BB;Blatz, JM;Choudhury, FA;Benjamin, D;Shohet, JL;Sussman, MR;
    Sci Rep
    test activity
  • A comparative analysis of EGFR-targeting antibodies for gold nanoparticle CT imaging of lung cancer
    Ashton, JR;Gottlin, EB;Patz, EF;West, JL;Badea, CT;
    PLoS ONE
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