CEACAM1 Protein, Human, Recombinant (ECD, His Tag)

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CEACAM1 Protein, Human, Recombinant (ECD, His Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Measured by its ability to bind biotinylated Human CEACAM6-His (Cat:10823-H08H) in functional ELISA.
2. Measured by its ability to bind biotinylated Human CEACAM8-His (Cat:11729-H08H) in functional ELISA.
3. Measured by its ability to inhibit IL­2 secretion by HuT 78 human cutaneous T cell lymphoma cells. The ED50 for this effect is typically 2­13 µg/mL.
Protein Construction
A DNA sequence encoding the extracellular domain of human CEACAM1 (NP_001020083.1) (Met 1-Gly 428) was expressed with a C-terminal polyhistidine tag.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 35
Molecule Mass
The secreted recombinant human CEACAM1 comprises 405 amino acids and predicts a molecular mass of 44.8 kDa. As a result of glycosylation, the apparent molecular mass of rhCEACAM1 is approximately 60.5 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CEACAM1 Protein, Human, Recombinant (ECD, His Tag): Images

Measured by its ability to inhibit IL­2 secretion by HuT 78 human cutaneous T cell lymphoma cells. The ED50 for this effect is typically 2­13 µg/mL.

CEACAM1 Protein, Human, Recombinant (ECD, His Tag): Synonyms

BGP Protein, Human; BGP1 Protein, Human; BGPI Protein, Human; CD66a Protein, Human; CEACAM‑1 Protein, Human

CEACAM1 Background Information

The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
Full Name
carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
References
  • Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.
  • Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.
  • Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
  • Thioloxidoreductase HP0231 of Helicobacter pylori impacts HopQ-dependent CagA translocation
    Author
    Grzeszczuk, MJ;Bocian-Ostrzycka, KM;Banaś, AM;Roszczenko-Jasinska, P;Malinowska, A;Stralova, H;Haas, R;Meyer, TF;Jagusztyn-Krynicka, EK;
    Year
    2018
    Journal
    Int. J. Med. Microbiol.
    Application
    binding
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