CD63 Protein, Human, Recombinant (His Tag)

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CD63 Protein, Human, Recombinant (His Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the second extracellular domain of human CD63 (P08962-1) (Ala 103-Val 203) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 103
Molecule Mass
The recombinant human CD63 consists of 112 amino acids after removal of the signal peptide and has a predicted molecular mass of 13 kDa. The apparent molecular mass of rh CD63 is approximately 20-25 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD63 Protein, Human, Recombinant (His Tag): Images

CD63 Protein, Human, Recombinant (His Tag): Synonyms

LAMP-3 Protein, Human; ME491 Protein, Human; MLA1 Protein, Human; OMA81H Protein, Human; TSPAN30 Protein, Human

CD63 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 63 (CD63) is a member of the CD family and the transmembrane 4 superfamily,also known as the tetraspanin family. CD63 is a cellular surface glycoprotein characterized by the presence of four bydrophobic domains. CD63 had functions in mediating signal transduction processes and then regulate variety of cellular processes such as cell proliferation, activation and motility. It has reported that CD63 protein associated with tumor progression and served as a blood platlet activation marker and the deficiency of this protein may be associated with Hermansky-Pudlak syndrome.
Full Name
CD63 molecule
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Radford KJ, et al. (1996) Associates with Transmembrane 4 Superfamily Members, CD9 and CD81, and with beta 1 Integrins in Human Melanoma. Biochemical Biophysical Research Communications. 222(1): 13-18.
  • Metzelaar MJ, et al. (1991) CD63 antigen, A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The journal of biological chemistry. 266: 3239-45.
  • Host protein CD63 enhances viral RNA replication by interacting with human astrovirus nonstructural protein nsP1a/4
    Author
    zhao, w;liu, N;Tao, X;Zheng, C;Li, X;Yu, M;Li, Y;
    Year
    2018
    Journal
    bioRxiv
    Application
    binding
  • Aptamer-based fluorescence polarization assay for separation-free exosome quantification
    Author
    Zhang, Z;Tang, C;Zhao, L;Xu, L;Zhou, W;Dong, Z;Yang, Y;Xie, Q;Fang, X;
    Year
    2019
    Journal
    Nanoscale
    Application
    binding (aptamer)
  • A novel aptamer LL4A specifically targets Vemurafenib-resistant melanoma through binding to the CD63 protein
    Author
    Li, H;Liu, J;Xiao, X;Sun, S;Zhang, H;Zhang, Y;Zhou, W;Zhang, B;Roy, M;Liu, H;Ye, M;Wang, Z;Liu-Smith, F;Liu, J;
    Year
    2019
    Journal
    Molecular Therapy - Nucleic Acids
    Application
    binding (aptamer)
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