CD48 Protein, Human, Recombinant, Biotinylated

1/1
Price:
Size:
Number:

CD48 Protein, Human, Recombinant, Biotinylated: Product Information

Purity
> 95 % as determined by SDS-PAGE.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Biotin/Protein Ratio
0.7-1 as determined by the HABA assay.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human CD48 (NP_001769.2) (Met1-Ser220) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln27
Molecule Mass
The recombinant human CD48 consists of 220 amino acids and predicts a molecular mass of 25.60 kDa. It migrates as an approximately 42.34 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD48 Protein, Human, Recombinant, Biotinylated: Images

CD48 Protein, Human, Recombinant, Biotinylated: Synonyms

BCM1 Protein, Human; BLAST Protein, Human; Blast-1 Protein, Human; BLAST1 Protein, Human; hCD48 Protein, Human; mCD48 Protein, Human; MEM-102 Protein, Human; SLAMF2 Protein, Human

CD48 Background Information

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
Full Name
CD48 molecule
Research Areas
References
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.