CD32B/Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated

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CD32B/Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Biotin/Protein Ratio
0.7-1 as determined by the HABA assay.
Activity
1. Measured by its ability to bind human IgG1 (Cat: 10702-HNAC) in functional ELISA.
2. Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated (Cat. 10259-H27H-B) bound to Avastin was 0.02 μM.
Protein Construction
A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 46
Molecule Mass
The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35 kDa protein due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD32B/Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Images

Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b (Cat: 10259-H27H-B). The EC50 of Human CD32b (Cat: 10259-H27H-B) is 1.6 - 3.7 μg/ml.
Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated (Cat. 10259-H27H-B) bound to Avastin was 0.02 μM.

CD32B/Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Synonyms

CD32 Protein, Human; CD32A Protein, Human; CD32B Protein, Human; CDw32 Protein, Human; Fc gamma RII Protein, Human; Fc gamma RIIB Protein, Human; FCG2 Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR21 Protein, Human; FCGR2A Protein, Human; IGFR2 Protein, Human

CD32B/Fcgr2b Background Information

Full Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • Enhanced Effector Functions Due to Antibody Defucosylation Depend on the Effector Cell Fcγ Receptor Profile
    Author
    Bruggeman, CW;Dekkers, G;Bentlage, AEH;Treffers, LW;Nagelkerke, SQ;Lissenberg-Thunnissen, S;Koeleman, CAM;Wuhrer, M;van den Berg, TK;Rispens, T;Vidarsson, G;Kuijpers, TW;
    Year
    2017
    Journal
    J. Immunol.
  • Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding
    Author
    Temming, AR;Bentlage, AEH;de Taeye, SW;Bosman, GP;Lissenberg-Thunnissen, SN;Derksen, NIL;Brasser, G;Mok, JY;van Esch, WJE;Howie, HL;Zimring, JC;Vidarsson, G;
    Year
    2020
    Journal
    Molecular immunology
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