CD32B/Fcgr2b Protein, Human, Recombinant (ECD, His & AVI Tag)

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CD32B/Fcgr2b Protein, Human, Recombinant (ECD, His & AVI Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Immobilized human CD32b-AVI-His (Cat:10259-H27H) at 10 μg/ml (100 μl/well) can bind biotinylated Human IgG1, The EC50 of biotinylated Human IgG1 is 0.20-0.48 μg/ml.
2. Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His & AVI Tag)(Cat: 10259-H27H) bound to Herceptin was 1.5 nM.
Protein Construction
A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 46
Molecule Mass
The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 30-35 kDa protein due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD32B/Fcgr2b Protein, Human, Recombinant (ECD, His & AVI Tag): Images

Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His & AVI Tag)(Cat: 10259-H27H) bound to Herceptin was 1.5 nM.

CD32B/Fcgr2b Protein, Human, Recombinant (ECD, His & AVI Tag): Synonyms

CD32 Protein, Human; CD32A Protein, Human; CD32B Protein, Human; CDw32 Protein, Human; Fc gamma RII Protein, Human; Fc gamma RIIB Protein, Human; FCG2 Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR21 Protein, Human; FCGR2A Protein, Human; IGFR2 Protein, Human

CD32B/Fcgr2b Background Information

Full Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
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