CD19 Protein, Human, Recombinant (ECD, His Tag)


CD19 Protein, Human, Recombinant (ECD, His Tag): Product Information

> 90 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
Immobilized Human CD19 His (Cat: 11880-H08H) at 2 μg/ml (100 μl/well) can bind Anti-CD19 Antibody (Cat: 11880-RP01), the EC50 of Anti-CD19 Antibody is 5-40 ng/mL.
Protein Construction
A DNA sequence encoding the human CD19 (P15391-1) extracellular domain (Met1-Lys291) was expressed with a polyhistidine tag at the C-terminus.
Expressed Host
HEK293 Cells
Predicted N Terminal
Pro 20
Molecule Mass
The recombinant human CD19 consists of 283 amino acids and predictes a molecular mass of 31.6 kDa. In SDS-PAGE under reducing conditions, rhCD19 migrates as an approximately 47 kDa band due to glycosylation.
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD19 Protein, Human, Recombinant (ECD, His Tag): Images

Immobilized Human CD19 His (Cat: 11880-H08H) at 2 μg/ml (100 μl/well) can bind Anti-CD19 Antibody (Cat: 11880-RP01), the EC50 of Anti-CD19 Antibody is 5-40 ng/mL.

CD19 Protein, Human, Recombinant (ECD, His Tag): Synonyms

B4 Protein, Human; CVID3 Protein, Human

CD19 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in the threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell co-receptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.
Full Name
CD19 molecule
Research Areas
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.
  • Chimeric antigen receptors that trigger phagocytosis
    Morrissey, M;Williamson, A;Steinbach, A;Roberts, E;Kern, N;Headley, M;Vale, R;
    Bead engulfment assay
  • Generation and characterization of avian-derived anti-human CD19 single chain fragment antibodies
    Tsai, KC;Chiang, CW;Lo, YN;Chang, FL;Lin, TY;Chang, CY;Chen, WC;Lee, YC;
    Anim. Biotechnol.
  • Imaging Chimeric Antigen Receptor (CAR) Activation
    Libby, KA;Su, X;
    Methods Mol. Biol.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.