c-MET Protein, Human, Recombinant (ECD, His Tag)
Cat: 10692-H08H
c-MET Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to bind human HGF (Cat: 10463-HNAS) in functional ELISA.
Protein Construction
A DNA sequence encoding the extracellular domain of human c-Met (NP_000236.2) (Met1-Thr932) was fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu 25
Molecule Mass
The mature form of recombinant human c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 919 amino acids and has a predicted molecular mass of 103 (α=33 +β=70) kDa. As a result of glycosylation, rh c-MET heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 85 kDa respectively in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.5
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
c-MET Protein, Human, Recombinant (ECD, His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 10 μg/ml (100 μl/well) can bind biotinylated c-Met. The EC50 of biotinylated c-Met is 5.28 μg/ml.
c-MET Protein, Human, Recombinant (ECD, His Tag): Synonyms
AUTS9 Protein, Human; c-Met Protein, Human; DFNB97 Protein, Human; HGFR Protein, Human; RCCP2 Protein, Human
c-MET Background Information
Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
Full Name
MET proto-oncogene, receptor tyrosine kinase
Research Areas
References
- McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
- Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
- Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
- Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
Human c-MET Proteins
Rat c-MET Proteins
Canine c-MET Proteins
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Anti-MET immunoPET for non-small cell lung cancer using fully human antibody fragmentsAuthorLi, K;Tavare, R;Zettlitz, KA;Mumenthaler, SM;Mallick, P;Zhou, Y;Marks, JD;Wu, AM;Year2014JournalMol. Cancer Ther.ApplicationPhage library selection
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Detection of colonic neoplasia in vivo using near-infrared-labeled peptide targeting cMetAuthorWu, X;Zhou, J;Wang, F;Meng, X;Chen, J;Chang, TS;Lee, M;Li, G;Li, X;Appelman, HD;Kuick, R;Wang, TD;Year2019JournalSci RepApplicationbiopanning
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Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumorsAuthorYuan, X;Sun, Z;Yuan, Q;Hou, W;Liang, Q;Wang, Y;Mo, W;Wang, H;Yu, M;Year2020JournalInvest New DrugsApplicationcell-based
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